Separation, purification and preliminary characterization of sulfated polysaccharides from Sargassum plagiophyllum and its in vitro anticancer and antioxidant activity

Suresh, Veeraperumal; Senthilkumar, N; Thangam, Ramar; Rajkumar, Mayan; Anbazhagan, C.; Rengasamy, Ramasamy; Gunasekaran, Palani; Kannan, Soundarapandian; Palani, Perumal

doi:10.1016/j.procbio.2012.12.014

Cited by 109 publications

(48 citation statements)

References 43 publications

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“…Bioactive polysaccharides containing abundant uronic acid contents have been isolated from several plants and algaes, including Cochlospermum tinctorium A. Rich, A. membranaceus and Sargassum plagiophyllum (Inngjerdingen et al 2013;Li et al 2014a, b, c;Suresh et al 2013). These results showed that the antitumor activity of APS2 with 48.87 % galacturonic acid was stronger than APS1 without galacturonic acid, and it is in accordance with previous results.…”

Section: Antitumor Activitysupporting

confidence: 86%

“…These results showed that the inhibition ratios of APS2 with Mw of 184.1 kDa were observably higher than that of APS1 with Mw of 125.4 kDa at the same concentration. Although the mechanism behind the biological activity of polysaccharides is not fully known, the monosaccharide composition also has an important influence on the antitumor activity of polysaccharides (Inngjerdingen et al 2013;Li et al 2014a, b, c;Suresh et al 2013). Bioactive polysaccharides containing abundant uronic acid contents have been isolated from several plants and algaes, including Cochlospermum tinctorium A.…”

Section: Antitumor Activitymentioning

confidence: 99%

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Ultrasound-assisted extraction of polysaccharides from Artemisia selengensis Turcz and its antioxidant and anticancer activities

Wang

Umair

et al. 2016

J Food Sci Technol

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Artemisia selengensis Turcz (AST) is a perennial herb with therapeutic and economic applications in China. The effects of ultrasound-assisted extraction (UAE) parameters upon extraction yield (EY%), antioxidant and antitumor activities of the polysaccharides extracts were studied by using a factorial design and response surface methodology. The optimal conditions determined were as: ultrasonic power 146 W, extraction time 14.5 min. and extraction temperature 60°C. The average molecular weights of two homogeneous polysaccharides (APS1 and APS2) purified by DEAE cellulose-52 and Sephadex G-100 column chromatography were 125.4 and 184.1 kDa, respectively. Monosaccharide analysis showed that APS1 and APS2 were composed of five common monomers i.e., galactose, mannose, arabinose, xylose and rhamnose and one different monomer glucose and galacturonic acid respectively, with a most abundant part in molar % of APS1 and APS2 were glucose (83.01 %) and galacturonic acid (48.87 %) while least were xylose (0.80 %) and mannose (1.73 %) respectively. The antioxidant properties were determined by evaluating DPPH, hydroxyl radical scavenging activity and reducing power which indicated both APS1 and APS2 showed strong scavenging activities and anticancer activities on HT-29, BGC823 and antitumor activity on HepG-2. As UAE improved the polysaccharides yield than CSE, meanwhile, no significant difference of polysaccharides chemical compositions. Therefore, the present study suggests that the consumption of AST leaves may beneficial for the treatment of many diseases.

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Section: Antitumor Activitysupporting

confidence: 86%

Section: Antitumor Activitymentioning

confidence: 99%

Ultrasound-assisted extraction of polysaccharides from Artemisia selengensis Turcz and its antioxidant and anticancer activities

Wang

Umair

et al. 2016

J Food Sci Technol

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“…That value was higher than IC 50 value from phlorotannin extract of S. polycystum was about 1.20±0.01 mg/ml, polyphenol extract of S. polycystum was about 1.27±0,01 mg/ ml (Cahyaningrum et al, 2016), water extract of S. boveanum was about 3.82 mg/ml (Zahra et al, 2007), sulphated polysaccharides of S. filipendula was about 1000 ppm (Costa et al, 2011), sulphated polysaccharides of Sargassum sp. was about 800 ppm (Ale et al, 2011), sulphated polysaccharides of Fucus vesiculosus was about 800 ppm (Suresh et al, 2013), sulphated polysaccharides of S. pallidum was about 1,000 ppm , and sulphated polysaccharides of S. plagiophyllum was about 700 ppm (Suresh et al, 2013). IC 50 value of S. hystrix dry powder was lower than sulphated polysaccharides of Ecklonia cava was about 43.9-100 ppm (Athukorala et al, 2009), sulphated polysaccharides of Cladosiphon okamuranus was about 100 ppm (Teruya et al, 2007), and S. hystrix extract was about 0.33±0.03 mg/ml (Budhiyanti et al, 2011).…”

Section: Antioxidant Activitymentioning

confidence: 96%

“…The value of IC 50 relatively strong if the value ranges between 50-100 ppm (Khotimah, 2013). However, Suresh et al (2013) mentioned that brown algae with the IC 50 value of 1000 ppm were potential to be used as in vitro anticancer. High and low IC 50 values are influenced by several factors, such as the solvents used, the amount of dissolved bioactive components (Molyneux, 2004), harvest seasons, harvest sites, and species (Budhiyanti et al, 2012).…”

Section: Antioxidant Activitymentioning

confidence: 99%

Antioxidant Activity and Proximate Analysis of Dry Powder from Brown Seaweed Sargassum hystrix

Lailatussifa

Husni

Isnansetyo

2017

JFS

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AbstrakPenelitian ini bertujuan untuk mengetahui aktivitas antioksidan, kandungan total fenol, kandungan nutrisi, dan kandungan fitokimia dari bubuk kering Sargassum hystrix. Ekstrak air dari sampel kering alga cokelat (S. hystrix) disiapkan dan diuji kandungan total fenolnya. Jumlah kandungan total fenol ekstrak air yaitu 11,43 g Gallic Acid Equivalent (GAE)/100 g berat kering sampel dengan menggunakan metode Folin-Ciocalteu. Aktivitas antioksidan (Radical Scavenging Activity/RSA) BHT yang digunakan sebagai standar tergolong tinggi, penghambatan radikal DPPH pada 1 mg sampel kering/ml pelarut yakni 96%. Nilai IC 50 BHT adalah sebesar 0,227 ± 0,001 mg sampel kering/ml pelarut, sedangkan aktivitas penangkapan radikal (RSA) bubuk kering S. hystrix sebesar 65,28% pada 1 mg sampel kering/ml pelarut. Nilai IC 50 bubuk kering S. hystrix adalah sebesar 0,616 ± 0,005 mg sampel kering/ml pelarut. Kandungan nutrisi dan komponen bioaktif bubuk kering S. hystrix seimbang untuk mendukung aktivitas antioksidan sampel. Hasil ini menunjukkan bahwa alga tersebut potensial sebagai sumber antioksidan alami. Kata kunci: Aktivitas antioksidan, bubuk kering Sargassum hystrix, fitokimia, kandungan fenolik, kandungan nutrisi AbstractThis research aims to know antioxidant activity, total phenolic, nutrient, and screening phytochemistry compounds of S. hystrix dry powder. Water extract from the dried sample of brown algae (S. hystrix) was prepared and examined for its phenolic compounds. The amount of total phenolic compounds in water extract was about 11.43 g Gallic Acid Equivalent (GAE)/100 g of dry basis sample, as measured by using Folin-Ciocalteu method. The antioxidant activity (Radical Scavenging Activity/RSA) of BHT as a standard was high about 96% inhibition of radical DPPH with 1 mg dry sample/ml solvent. The IC 50 of the BHT was 0.227 ± 0.001 mg of dry sample/ ml solvent. While the radical activity (RSA) of S. hystrix dry powder was about 65.28% at 1 mg dry sample/ml solvent. The IC 50 of S. hystrix dry powder was 0.616 ± 0.005 mg of dry sample/ml solvent. Nutrient contents and bioactive compounds of dry powder S. hystrix were balanced to support antioxidant activity of the sample. The results suggest that this alga is a potential source of natural antioxidant.

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“…It has been used as an antiseptic, and as an anti-diuretic for urinary disorders. A variety of research has shown that it exhibits a wide range of biological effects, including a skeletal muscle relaxant effect [4], analgesic and anti-inflammatory effects [5], antifungal activity [6] and an antifertility effect [7]. Purslane has also shown other beneficial effects such as antidiabetic [8] and wound healing properties [9].…”

Section: Introductionmentioning

confidence: 99%

Extraction, purification and antibacterial activity of polysaccharides from Purslane

Gu¹,

Shu²

2017

Proceedings of the 2017 6th International Conference on Energy, Environment and Sustainable Development (ICEESD 2017)

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Abstract. In this study, the polysaccharides from Purslane were extracted by ultrasonic method and its content was measured by the phenol-sulfuric acid method using glucose as the standard. The crude polysaccharides were collected and purified by DEAE-Cellulose anion-exchange and Sephadex gel-permeation chromatography. Three fractions were obtained and named POL1, POL2 and POL3, respectively. The molecular weights of these three fractions were measured by Sephadex using dextran standard curve. Escherichia coli, Acetic acid bacteria, Saccharomycetes and Aspergillus Niger were used to study the antibacterial activity of the polysaccharides. The results showed that molecular weights of POL1, POL2 and POL3 were 18Kd, 55kd and 108kd, respectively. The polysaccharides had inhibitory effect on microorganisms, and the inhibited order of microorganisms was: Saccharomycetes>Acetic acid bacteria>Aspergillus Niger>Escherichia coli. In addition, the order of antibacterial activities of these three fractions on microorganisms was: POL3>POL2b>POL1b. It could be concluded that three fractions were obtained by purification. These three fractions had inhibitory effect on Saccharomycetes, Acetic acid bacteria, Aspergillus Niger and Escherichia coli. The antibacterial activity of polysaccharides from Purslane against Saccharomycetes was the strongest, while the weakest against Escherichia coli. In addition, POL3 was found to be the most effective against the tested microorganisms among the three fractions.

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Separation, purification and preliminary characterization of sulfated polysaccharides from Sargassum plagiophyllum and its in vitro anticancer and antioxidant activity

Cited by 109 publications

References 43 publications

Ultrasound-assisted extraction of polysaccharides from Artemisia selengensis Turcz and its antioxidant and anticancer activities

Ultrasound-assisted extraction of polysaccharides from Artemisia selengensis Turcz and its antioxidant and anticancer activities

Antioxidant Activity and Proximate Analysis of Dry Powder from Brown Seaweed Sargassum hystrix

Extraction, purification and antibacterial activity of polysaccharides from Purslane

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