Ultrasound-assisted extraction of polysaccharides from Artemisia selengensis Turcz and its antioxidant and anticancer activities

Abstract

Artemisia selengensis Turcz (AST) is a perennial herb with therapeutic and economic applications in China. The effects of ultrasound-assisted extraction (UAE) parameters upon extraction yield (EY%), antioxidant and antitumor activities of the polysaccharides extracts were studied by using a factorial design and response surface methodology. The optimal conditions determined were as: ultrasonic power 146 W, extraction time 14.5 min. and extraction temperature 60°C. The average molecular weights of two homogeneous polysaccharides (APS1 and APS2) purified by DEAE cellulose-52 and Sephadex G-100 column chromatography were 125.4 and 184.1 kDa, respectively. Monosaccharide analysis showed that APS1 and APS2 were composed of five common monomers i.e., galactose, mannose, arabinose, xylose and rhamnose and one different monomer glucose and galacturonic acid respectively, with a most abundant part in molar % of APS1 and APS2 were glucose (83.01 %) and galacturonic acid (48.87 %) while least were xylose (0.80 %) and mannose (1.73 %) respectively. The antioxidant properties were determined by evaluating DPPH, hydroxyl radical scavenging activity and reducing power which indicated both APS1 and APS2 showed strong scavenging activities and anticancer activities on HT-29, BGC823 and antitumor activity on HepG-2. As UAE improved the polysaccharides yield than CSE, meanwhile, no significant difference of polysaccharides chemical compositions. Therefore, the present study suggests that the consumption of AST leaves may beneficial for the treatment of many diseases.

Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities

“…To address these problems, microwaveassisted extraction [15] and supercritical fluid extraction [20] have been developed for the extraction of flavonoids [15]. Although those methods efficiently extract flavonoids from plant materials, the microwave-assisted extraction is difficult to scale up and requires high microwave power, which increases energy consumption [15,21], whereas the supercritical method proceeds at high pressure (48 MPa), which requires expensive reactor and rises a safety concern [20]. Enzyme-assisted extraction has been proposed as another alternative for the flavonoids extraction because this process was performed at mild extraction conditions [22,23].…”

“…In recent years, the ultrasound-assisted process has gained increased interest in the phytochemical extraction due to its rapid and high extraction efficiency [21,24]. is method employs ultrasonication, which is ultrasonic waves, to disrupt a cell wall through inducing cavitation in the solvent [21].…”

“…In recent years, the ultrasound-assisted process has gained increased interest in the phytochemical extraction due to its rapid and high extraction efficiency [21,24]. is method employs ultrasonication, which is ultrasonic waves, to disrupt a cell wall through inducing cavitation in the solvent [21]. is method enhances the mass transfer and solvent penetration into the plant materials, which promote the release of soluble compounds from the plant cell into the extracting solvent [21,25].…”

“…is method employs ultrasonication, which is ultrasonic waves, to disrupt a cell wall through inducing cavitation in the solvent [21]. is method enhances the mass transfer and solvent penetration into the plant materials, which promote the release of soluble compounds from the plant cell into the extracting solvent [21,25]. Consequently, this method shortens the extraction time and enhances the extraction efficiency [24].…”

Compared extraction methods on the physicochemical properties, antioxidant activity, and optimization of enzyme‐assisted extraction of polysaccharides from Gynura medica

“…UEAE is an assisted extraction method combined ultrasonic with enzyme, which is undoubtedly an emerging technology in the food industry since it owns advantages of two extraction methods such as mild extraction conditions, lower investment costs and energy requirements, and simplified manipulation. It is an effective method for polysaccharides extraction that has a higher yield and bioactivity (Wang et al, ). Given this, it is necessary to compare the various extraction methods on Gynura medica polysaccharides.…”