Separation ofPodophyllum lignans by micellar electrokinetic capillary chromatography (MECC)

Ganzera, Markus; Moraes, Rita M.; Khan, Ikhlas A.

doi:10.1007/bf02467758

Cited by 12 publications

(5 citation statements)

References 5 publications

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“…Thus far no analytical procedure has been established to study the lignan composition of A. sylvestris rhizomes. Various analytical procedures for aryltetralins and other groups of lignans have been published like HPLC (13), GC (3), (14), and MECC (15). These are all quantitative methods, some of which are applied on plant material.…”

Section: Resultsmentioning

confidence: 99%

A Fast and Simple GC MS Method for Lignan Profiling in Anthriscus sylvestris and Biosynthetically Related Plant Species

Koulman

Bos

Medarde³

et al. 2001

Planta med

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A new GC-MS method for monitoring lignans was developed to study the variation in plants and elucidate the biosynthetic steps. A simple and fast extraction procedure for lyophilised plant material was developed, giving a lignan-rich extract. A GC-MS method was set up using an apolar WCOT fused silica column using a high temperature programme (150 degrees C to 320 degrees C at 15 degrees C min(-1)). This new GC-MS method gave a clear lignan profile of plant material. It was possible to show the large variation in the concentrations of deoxypodophyllotoxin (DOP), yatein and anhydropodorhizol (AHP) in Anthriscus sylvestris (L.) Hoffm. plants growing on different locations using cinchonidin as an internal standard. In contrast with existing GC methods for lignan analysis no derivatisation is needed. It is also possible to use this method for the detection of different classes of lignans in biosynthetically related plant species.

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Section: Resultsmentioning

confidence: 99%

A Fast and Simple GC MS Method for Lignan Profiling in Anthriscus sylvestris and Biosynthetically Related Plant Species

Koulman

Bos

Medarde³

et al. 2001

Planta med

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“…Since then several studies have been performed on MEKC separation of Podophyllum lignans. The main lignans including their glucoside derivatives were separated by Ganzera et al using 25 mM sodium dodecylsulfate (SDS) and 15% methanol in 40 mM phosphate-15 mM tetraborate (pH 7.25) as a background electrolyte [106]. The method was applied to compare different samples of Podophyllum peltatum collected in various part of USA, however just one lignan-podophyllotoxin was quantify.…”

Section: Micellar Electrokinetic Capillary Chromatographymentioning

confidence: 99%

Separation procedures applicable to lignan analysis

Slanina

Glatz

2004

Journal of Chromatography B

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“…1 Several analytical techniques have been reported for the quantitative determination of Be.Cl in pure solutions and pharmaceutical preparations. Among these are HPLC, [2][3][4][5][6][7] TLC, 8 capillary electrophoresis, determination of ions in solution with high selectivity. Most ISE's are of low-cost, their use and maintenance being very simple, assay procedures involving such electrodes are generally simple and fast.…”

Section: Introductionmentioning

confidence: 99%

Potentiometric Batch and Flow Injection Analysis of Betaine Hydrochloride

2007

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Novel PVC membrane electrodes for the determination of betaine ion based on the formation of betaine-tetraphenylborate (Be-TPB) and betaine-phosphotungstate (Be-PT) ion-exchangers as electroactive materials are described. The sensors show a fast, stable, near Nernstian response for 6.92 x 10(-6) to 7.94 x 10(-3) M and 1.0 x 10(-4) to 1.0 x 10(-2) M betaine hydrochloride (Be.Cl) in case of Be-TPB electrode applying batch and flow injection analysis (FIA), respectively, and 2.95 x 10(-5) to 2.26 x 10(-3) M and 3.16 x 10(-5) to 1.0 x 10(-2) M in case of Be-PT electrode for batch and FIA electrodes, respectively, at 25 degrees C over the pH range of 3.5-10 with a cationic slope of 60.2 and 59.1 mV decade(-1) and a fast potential response of < or =15 s. The lower detection limits are 7.94 x 10(-6) and 3.18 x 10(-5) M Be.Cl for Be-TPB and Be-PT electrodes, respectively. Selectivity coefficient data for some common inorganic cations, sugars, amino acids and the components other than betaine, of the mixed drug investigated show negligible interference. The electrodes have been applied to the direct potentiometric determination of betaine hydrochloride in water and in a pharmaceutical preparation under batch and FIA conditions. Potentiometric titrations of Be.Cl with NaTPB and PTA as titrants were monitored with the developed betaine electrodes as an end point indicator electrode. The determination of Be.Cl shows an average recovery of 100.8% with mean relative standard deviation of 0.61%. The effect of temperature on the electrodes was also studied.

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Separation ofPodophyllum lignans by micellar electrokinetic capillary chromatography (MECC)

Cited by 12 publications

References 5 publications

A Fast and Simple GC MS Method for Lignan Profiling in Anthriscus sylvestris and Biosynthetically Related Plant Species

A Fast and Simple GC MS Method for Lignan Profiling in Anthriscus sylvestris and Biosynthetically Related Plant Species

Separation procedures applicable to lignan analysis

Potentiometric Batch and Flow Injection Analysis of Betaine Hydrochloride

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