A Fast and Simple GC MS Method for Lignan Profiling in Anthriscus sylvestris and Biosynthetically Related Plant Species

Abstract

A new GC-MS method for monitoring lignans was developed to study the variation in plants and elucidate the biosynthetic steps. A simple and fast extraction procedure for lyophilised plant material was developed, giving a lignan-rich extract. A GC-MS method was set up using an apolar WCOT fused silica column using a high temperature programme (150 degrees C to 320 degrees C at 15 degrees C min(-1)). This new GC-MS method gave a clear lignan profile of plant material. It was possible to show the large variation in the concentrations of deoxypodophyllotoxin (DOP), yatein and anhydropodorhizol (AHP) in Anthriscus sylvestris (L.) Hoffm. plants growing on different locations using cinchonidin as an internal standard. In contrast with existing GC methods for lignan analysis no derivatisation is needed. It is also possible to use this method for the detection of different classes of lignans in biosynthetically related plant species.

Deoxypodophyllotoxin Content and Antioxidant Activity of Aerial Parts of Anthriscus sylvestris Hoffm.

“…These various factors can have an impact on the secondary metabolite profile in higher plants (Harborne, 1982). A large variation in the contents of lignans in A. sylvestris plants growing at different locations was also described by Koulman et al (2001). The deoxypodophyllotoxin content of roots was higher (about 50%) than that found in the aerial parts of two different populations of A. sylvestris.…”

“…However, the isolation of podophyllotoxin from the rhizomes of Podophyllum plants is not a very ideal system for large-scale production and the supply becomes increasingly limited due to the intensive collection (Choudhary et al, 1998). For this reason different authors are searching for alternative sources of podophyllotoxin (Van Uden et al, 1997;Koulman et al, 2001). Because of their content of deoxypodophyllotoxin the rhizomes of A. sylvestris could become a valuable source for the bio-0939Ð5075/2006/0900Ð0658 $ 06.00 " 2006 Verlag der Zeitschrift für Naturforschung, Tübingen · http://www.znaturforsch.com · D technological production of podophyllotoxin (Van Uden et al, 1997).…”

Comparison of different extraction methods for the determination of podophyllotoxin and 6-methoxypodophyllotoxin in Linum species

“…Different extraction methods have been published for the extraction of podophyllotoxin from plant material [1,4,12,13]. Commercial production of podophyllotoxin involves its purification from an ethanolic extract of the dried roots and rhizomes of P. emodii.…”

Biosynthesis of Gold and Silver Nanoparticles with Anti-Microbial Activity by Callus Cultures of <I>Michelia champaca</I> L.

“…Lignans were isolated as described by Koulman et al 21 using 4 wk old callus cultures. After TLC analysis of the dichloromethane fraction of the methanolic extract when the plates were visualized under UV, 5 spots could be detected with R f values of 0.8, 0.75, 0.65, 0.3 and 0.25 ( Fig.…”