Separation of white blood cells from erythrocytes on a dielectrophoresis (DEP) based ‘Lab-on-a-chip’ device

Borgatti, Monica; Altomare, Luigi; Baruffa, M.; Fabbri, Enrica; Breveglieri, Giulia; Feriotto, Giordana; Manaresi, Nicolò; Medoro, Gianni; Romani, Annalisa; Tartagni, Marco; Gambari, Roberto; Guerrieri, R.

doi:10.3892/ijmm.15.6.913

Cited by 28 publications

(23 citation statements)

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“…DEP has been used to distinguish viable from nonviable cells , bacteria , malaria‐infected cells , leukocytes from RBCs , yeast cells , virus , DNA , and proteins . It has also been used to separate or characterize several nonbiological particles, such as polystyrene particles and colloids .…”

Section: Methodsmentioning

confidence: 99%

Dielectrophoretic separation of bioparticles in microdevices: A review

2014

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In recent years, dielectrophoretic force has been used to manipulate colloids, inert particles, and biological microparticles, such as red blood cells, white blood cells, platelets, cancer cells, bacteria, yeast, microorganisms, proteins, DNA, etc. This specific electrokinetic technique has been used for trapping, sorting, focusing, filtration, patterning, assembly, and separating biological entities/particles suspended in a buffer medium. Dielectrophoretic forces acting on particles depend on various parameters, for example, charge of the particle, geometry of the device, dielectric constant of the medium and particle, and physiology of the particle. Therefore, to design an effective micro-/nanofluidic separation platform, it is necessary to understand the role of the aforementioned parameters on particle motion. In this paper, we review studies particularly related to dielectrophoretic separation in microfluidic devices. Both experimental and theoretical works by several researchers are highlighted in this article covering AC and DC DEP. In addition, AC/DC DEP, which uses a combination of low frequency AC and DC voltage to manipulate bioparticles, has been discussed briefly. Contactless DEP, a variation of DC DEP in which electrodes do not come in contact with particles, has also been reviewed. Moreover, dielectrophoretic force-based field flow fractionations are featured to demonstrate the bioparticle separation in microfluidic device. In numerical front, a comprehensive review is provided starting from the most simplified effective moment Stokes-drag (EMSD) method to the most advanced interface resolved method. Unlike EMSD method, recently developed advanced numerical methods consider the size and shape of the particle in the electric and flow field calculations, and these methods provide much more accurate results than the EMSD method for microparticles.

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Section: Methodsmentioning

confidence: 99%

Dielectrophoretic separation of bioparticles in microdevices: A review

2014

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“…In another non-FFF method, slim and planar interdigitated electrode arrays are used for lateral separation of particles or cells Zhou et al 2005;Borgatti et al 2005b;Vulto et al 2006;Chen et al 2007;Fabbri et al 2008;Zou et al 2008). Compared with the conventional castellated electrode layout, this design is easier for fabrication and the separated particle aggregations are more concentrated and potentially easier for collection and detection.…”

Section: Separationmentioning

confidence: 98%

Electrokinetic motion of particles and cells in microchannels

Kang

2009

Microfluid Nanofluid

186

135

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This paper provides an overview of the electrokinetic phenomena associated with particles and cells in microchannel systems. The most important phenomena covered include electrophoresis, dielectrophoresis, and induced-charge electrokinetics. The latest development of these electrokinetic techniques for particle or cell manipulations in microfluidic systems is reviewed, in terms of the basic theories, mathematical models, numerical and experimental methods, and the key results/findings from the published literatures in the most recent decades. Some of the limitations associated with the negative field effects are discussed and the perspectives for the future investigations are summarized.

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“…Using a spiral electrode configuration, the dielectrophoretic movement of cells based on health was studied and malarial cells were separated from healthy cells 6. DEP has also been used to separate white blood cells from erythrocytes 24 and sample preparation for chip‐based hybridization assays in an integrated DNA or RNA system 7. Furthermore, dielectrophoretic field flow fractionation was used to study differential analysis of human leukocytes 25.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic characterization of erythrocytes: Positive ABO blood types

et al. 2008

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Dielectrophoretic manipulation of erythrocytes/red blood cells is investigated as a tool to identify blood type for medical diagnostic applications. Positive blood types of the ABO typing system (A+, B+, AB+ and O+) were tested and cell responses quantified. The dielectrophoretic response of each blood type was observed in a platinum electrode microdevice, delivering a field of 0.025V(pp)/microm at 1 MHz. Responses were recorded via video microscopy for 120 s and erythrocyte positions were tabulated at 20-30 s intervals. Both vertical and horizontal motions of erythrocytes were quantified via image object recognition, object tracking in MATLAB, binning into appropriate electric field contoured regions (wedges) and statistical analysis. Cells of O+ type showed relatively attenuated response to the dielectrophoretic field and were distinguished with greater than 95% confidence from all the other three blood types. AB+ cell responses differed from A+ and B+ blood types likely because AB+ erythrocytes express both the A and B glycoforms on their membrane. This research suggests that dielectrophoresis of untreated erythrocytes beyond simple dilution depends on blood type and could be used in portable blood typing devices.

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Separation of white blood cells from erythrocytes on a dielectrophoresis (DEP) based ‘Lab-on-a-chip’ device

Cited by 28 publications

References 0 publications

Dielectrophoretic separation of bioparticles in microdevices: A review

Dielectrophoretic separation of bioparticles in microdevices: A review

Electrokinetic motion of particles and cells in microchannels

Dielectrophoretic characterization of erythrocytes: Positive ABO blood types

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