Dielectrophoretic characterization of erythrocytes: Positive ABO blood types

Srivastava, S. D.; Daggolu, Prashant R.; Burgess, Shane C.; Minerick, Adrienne R.

doi:10.1002/elps.200800166

Cited by 75 publications

(82 citation statements)

References 27 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When working on the microscale, processing times are much shorter, only small quantities of samples and reagents are required, higher resolution and sensitivity are obtained, high level of integration, portability, and automation are achieved, and costs are lowered [4,6]. Microfluidic devices have made an impact in many fields including food and water safety [7][8][9], clinical analysis [10][11][12], medical diagnostics [12,13], DNA [14][15][16][17] and protein manipulation [15,18,19], and environmental monitoring [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic monitoring of microorganisms in environmental applications

Jesús‐Pérez¹,

Lapizco‐Encinas²

2011

Electrophoresis

View full text Add to dashboard Cite

Dielectrophoresis (DEP) is the motion of polarizable particles in the presence of nonuniform electric fields. This novel electrokinetic technique has successfully been employed in many miniaturized systems for the manipulation and detection of microbes. This review article depicts the application of dielectrophoresis for the monitoring of microorganisms in microfluidic devices for environmental applications. The research studies described here are mainly conceived for water- and air-monitoring assessments, and are classified considering the target aimed to detect, concentrate, and/or separate, including chemical and toxicant agents, and microorganisms ranging from virus to protozoa. Dielectrophoresis has also played an important role in biofilm formation studies. This review article comprises mainly studies published from 2000 to present. Even in this relatively short time frame, there have been many significant contributions of this powerful and nascent technique related to environmental monitoring; thus, unveiling its great potential for future research directions.

show abstract

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic monitoring of microorganisms in environmental applications

Jesús‐Pérez¹,

Lapizco‐Encinas²

2011

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…Results demonstrate that a 3D laminated graphene paper-based device can illustrate similar dielectrophoretic signatures as 3D metal foil laminated devices 20,21 , traditional 2D metal-electrode 26,27 , and 2D insulator devices 25 . In the following experiments, an 15 V peak-peak AC signal was applied and frequency was varied from 100 Hz to 10 MHz 30 .…”

Section: Representative Resultsmentioning

confidence: 78%

“…Advantages of utilizing graphene paper are versatility of physical and chemical properties, reduced expense, and the graphene nanoplatelets can concurrently act as biosensors to detect a wide range of bioanalytes 24 . Long-term goals of high throughput 3D DEP systems are to rapidly identify cell types [25][26][27] , or achieve label-free, electrically mediated cell sorting of diseased cells from populations of healthy cells 28 . This paper demonstrates material optimization and device preparation and operation followed by illustration and analysis of typical results.…”

Section: Introductionmentioning

confidence: 99%

Development of a 3D Graphene Electrode Dielectrophoretic Device

Xie

Tewari

Fukushima

et al. 2014

JoVE

Self Cite

View full text Add to dashboard Cite

The design and fabrication of a novel 3D electrode microdevice using 50 µm thick graphene paper and 100 µm double sided tape is described. The protocol details the procedures to construct a versatile, reusable, multiple layer, laminated dielectrophoresis chamber. Specifically, six layers of 50 µm x 0.7 cm x 2 cm graphene paper and five layers of double sided tape were alternately stacked together, then clamped to a glass slide. Then a 700 μm diameter micro-well was drilled through the laminated structure using a computer-controlled micro drilling machine. Insulating properties of the tape layer between adjacent graphene layers were assured by resistance tests. Silver conductive epoxy connected alternate layers of graphene paper and formed stable connections between the graphene paper and external copper wire electrodes. The finished device was then clamped and sealed to a glass slide. The electric field gradient was modeled within the multi-layer device. Dielectrophoretic behaviors of 6 μm polystyrene beads were demonstrated in the 1 mm deep micro-well, with medium conductivities ranging from 0.0001 S/m to 1.3 S/m, and applied signal frequencies from 100 Hz to 10 MHz. Negative dielectrophoretic responses were observed in three dimensions over most of the conductivity-frequency space and cross-over frequency values are consistent with previously reported literature values. The device did not prevent AC electroosmosis and electrothermal flows, which occurred in the low and high frequency regions, respectively. The graphene paper utilized in this device is versatile and could subsequently function as a biosensor after dielectrophoretic characterizations are complete. Video LinkThe video component of this article can be found at

show abstract

“…It involves the manipulation of particles in a non-uniform electric field based on the physical and electrical properties of the particle. DEP has been used for sorting of many biological samples such as bacteria [68,83,84], DNA [155][156][157], and red blood cells [158][159][160]. Although DEP is effective for cell isolation, it is often coupled with other techniques to provide quantitative and qualitative information.…”

Section: Introductionmentioning

confidence: 99%

The use of microfluidics and dielectrophoresis for separation, concentration, and identification of bacteria

et al. 2016

View full text Add to dashboard Cite

Traditional bacterial analyses take one to two days under favorable conditions where the bulk of the time is spent waiting for bacteria to divide and grow until visual colonies can be observed for identification. In the case of bacteria with slow doubling times, this process can take weeks. This delay in analysis is unacceptable, especially in cases of life threatening diseases or emergencies. It is clear that in order to decrease the analysis time of the bacteria, the culturing and growth step must be circumvented. The goal of this research is to design, build, and test a device that could decrease the analysis time of bacteria using label-free methods of dielectrophoresis and Raman spectroscopy.Testing for device design was performed with clinical samples in mind, which consist of bacteria grown in a variety of environmental conditions (i.e. available food sources, growth stage, temperature, etc.) and accompanied by sample debris. Raman spectra of bacteria grown in varying media and metabolic stages were collected and analyzed. Results indicate that growth phase and media have an impact on Raman spectra iv and is distinguishable by linear discriminant analysis (LDA). Despite these spectral differences, it was found that LDA classification of closely related bacteria remains fairly high (90%) regardless of growth phase. Sample debris were also considered in device design and accommodated for by dielectrophoresis. Devices were built with the goal to isolate bacteria from a mixed sample and simultaneously acquire Raman spectra for identification.For this dissertation, a device was designed, built, and tested that incorporates dielectrophoresis for particle isolation and Raman spectroscopy for identification. The device was modeled in COMSOL to ensure that an appropriate electrical field gradient could be obtained to isolate bacteria from 5 µm diameter polystyrene spheres. The device was built and successfully trapped bacteria away from polystyrene spheres and Raman spectra of the bacteria were collected while trapped. These results indicate a clear potential for contactless dielectrophoresis-Raman devices to isolate and identify bacteria from sample debris, and thereby decrease the analysis time of bacteria. Typical bacterial analysis involves culturing and visualizing colonies on an array of agar plates. The growth patterns and colors among the array are used to identify the bacteria. For fast growing bacteria such as Escherichia coli, analysis will take one to two days. However, slow growing bacteria such as mycobacteria can take weeks to identify.In addition, there are some species of bacteria that are viable but nonculturable. This lengthy analysis time is unacceptable for life-threatening infections and emergency situations. It is clear that to decrease the analysis of the bacteria, the culturing and growth steps must be avoided. The goal of this research is to design, build, and test a device that could decrease the analysis time of bacteria.

show abstract

Dielectrophoretic characterization of erythrocytes: Positive ABO blood types

Cited by 75 publications

References 27 publications

Dielectrophoretic monitoring of microorganisms in environmental applications

Dielectrophoretic monitoring of microorganisms in environmental applications

Development of a 3D Graphene Electrode Dielectrophoretic Device

The use of microfluidics and dielectrophoresis for separation, concentration, and identification of bacteria

Contact Info

Product

Resources

About