Separation of two monoamine oxidases from bovine bran

McCauley, Roy; Racker, Efraim

doi:10.1007/bf01659940

Cited by 128 publications

(33 citation statements)

References 13 publications

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“…Antibody and Immunoblotting-An antibody against purified Tm-GRP was raised by injecting 10 g of the purified protein into a male albino rabbit with complete Freund's adjuvant and giving two booster injections with the same amount of protein 7 and 14 days later (45). The resulting antibody was affinity-purified as previously described (46).…”

Section: Methodsmentioning

confidence: 99%

Characterization and Properties of a 1,3-β-d-Glucan Pattern Recognition Protein of Tenebrio molitor Larvae That Is Specifically Degraded by Serine Protease during Prophenoloxidase Activation

Zhang

Cho

Kim

et al. 2003

Journal of Biological Chemistry

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Although many different pattern recognition receptors recognizing peptidoglycan and 1,3-␤-D-glucan have been identified in vertebrates and insects, the molecular mechanism of these molecules in the pattern recognition and subsequent signaling is largely unknown. To gain insights into the action mechanism of 1,3-␤-D-glucan pattern recognition protein in the insect prophenoloxidase (proPO) activation system, we purified a 53-kDa 1,3-␤-D-glucan recognition protein (Tm-GRP) to homogeneity from the hemolymph of the mealworm, Tenebrio molitor, by using a 1,3-␤-D-glucan affinity column. The purified protein specifically bound to 1,3-␤-Dglucan but not to peptidoglycan. Subsequent molecular cloning revealed that Tm-GRP contains a region with close sequence similarity to bacterial glucanases. Strikingly, two catalytically important residues in glucanases are replaced with other nonhomologous amino acids in Tm-GRP. The finding suggests that Tm-GRP has evolved from an ancestral gene of glucanases but retained only the ability to recognize 1,3-␤-D-glucan. A Western blot analysis of the protein level of endogenous Tm-GRP showed that the protein was specifically degraded following the activation of proPO with 1,3-␤-Dglucan and calcium ion. The degradation was significantly retarded by the addition of serine protease inhibitors but not by cysteine or acidic protease inhibitor. These results suggest that 1,3-␤-D-glucan pattern recognition protein is specifically degraded by serine protease(s) during proPO activation, and we propose that this degradation is an important regulatory mechanism of the activation of the proPO system.

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Section: Methodsmentioning

confidence: 99%

Characterization and Properties of a 1,3-β-d-Glucan Pattern Recognition Protein of Tenebrio molitor Larvae That Is Specifically Degraded by Serine Protease during Prophenoloxidase Activation

Zhang

Cho

Kim

et al. 2003

Journal of Biological Chemistry

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“…Antibody and Immunoblotting-Antibodies against the purified Hd-PGRP-1 and Hd-PGRP-2 were raised by injecting 10 g of the purified proteins into a male albino rabbit with complete Freund's adjuvant and twice giving booster injections with the same amount of protein 7 and 14 days later (33). The resulting antibodies were affinity-purified as previously described (14).…”

Section: 3-␤-d-glucan and Pgn Binding Assay-mentioning

confidence: 99%

Peptidoglycan Recognition Proteins Involved in 1,3-β-D-Glucan-dependent Prophenoloxidase Activation System of Insect

Lee

Osaki

Lee

et al. 2004

Journal of Biological Chemistry

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“…IgG was purified from sera as in [4]. The Fc portion of this IgG was digested with pepsin (Worthington, Freehold, NJ) and the F(ab')s portion was purified by Sephadex G-l 50 column chromatography as in [5].…”

Section: Methodsmentioning

confidence: 99%

Fc‐receptor modulates the fusion between phagocytic vesicles and lysosomes in guinea pig polymorphonuclear leukocytes

Amano

Mizuno

1980

FEBS Letters

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Separation of two monoamine oxidases from bovine bran

Cited by 128 publications

References 13 publications

Characterization and Properties of a 1,3-β-d-Glucan Pattern Recognition Protein of Tenebrio molitor Larvae That Is Specifically Degraded by Serine Protease during Prophenoloxidase Activation

Characterization and Properties of a 1,3-β-d-Glucan Pattern Recognition Protein of Tenebrio molitor Larvae That Is Specifically Degraded by Serine Protease during Prophenoloxidase Activation

Peptidoglycan Recognition Proteins Involved in 1,3-β-D-Glucan-dependent Prophenoloxidase Activation System of Insect

Fc‐receptor modulates the fusion between phagocytic vesicles and lysosomes in guinea pig polymorphonuclear leukocytes

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