Fc‐receptor modulates the fusion between phagocytic vesicles and lysosomes in guinea pig polymorphonuclear leukocytes

Amano, Fumio; Mizuno, Den′ichi

doi:10.1016/0014-5793(80)81166-5

Cited by 7 publications

(1 citation statement)

References 6 publications

(8 reference statements)

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“…(2) An oil-droplet preparation consisting of 1,2,3,6tetrahydrophthalic acid di-isodecyl ester and immunoglobulin G was used, and phagocytosis was carried out in the presence of cyanide. Opsonization with immunoglobulin G stimulates the vesicle formation and enhances the yield of the vesicle fraction (Amano & Mizuno, 1980). The tetrahydrophthalic acid ester has suitable physical properties for separating the cells from the oil droplets and the vesicles from other particulate components.…”

Section: Discussionmentioning

confidence: 99%

Superoxide-forming NADPH oxidase preparation of pig polymorphonuclear leucocyte

Wakeyama¹,

Takeshige²,

Takayanagi³

et al. 1982

Biochemical Journal

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A phagocytic vesicle fraction with high NADPH-dependent superoxide-forming activity was obtained in large quantity from pig blood polymorphonuclear leucocytes, phagocytosing oil droplets in the presence of cyanide. The activity of the homogenate of the phagocytosing cells was 40 times that of the resting cells, and 70% of the activity in the homogenate was recovered in the phagocytic vesicle fraction. Essentially all of the superoxide-forming activity was extracted by repeated extraction with a mixture containing deoxycholate and Tween 20. The extract had a superoxide-forming activity of 1 mumol/min per mg of protein with NADPH, and one-fifth of this with NADH, Km values being similar to those of the vesicle fraction (40 microM for NADPH and 400 microM for NADH). A stoichiometric relationship of 1:2 for NADPH oxidation and superoxide formation was obtained, in agreement with the reaction NADPH +2O2 leads to NADP+ + 2O2 -. + H+. The activity of the extract was enhanced 2-fold by the addition of FAD, suggesting that the flavin is a component of the enzyme system. The Km value for FAD was 0.077 microM. The activities in both vesicle fraction and extract were labile even on refrigeration, but could be kept for several months at -70 degrees C.

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Section: Discussionmentioning

confidence: 99%

Superoxide-forming NADPH oxidase preparation of pig polymorphonuclear leucocyte

Wakeyama¹,

Takeshige²,

Takayanagi³

et al. 1982

Biochemical Journal

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Molecular Aspects of Membrane Fusion

Lucy¹

1984

Membrane Processes

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Fc receptor—More answers, more questions

Fornůsek

Větvička

1984

Folia Microbiol

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Fc receptors, belonging to the most important surface structures of a number of cells participating in the immune processes, have been intensely studied during the past decade. The present review summarizes the contemporary knowledge of the specificity and heterogeneity of Fc receptors and of factors influencing their expression, and includes some views on their function. In addition, it mentions their relationship to other cell surface structures, expression of Fc receptors during ontogeny of the organism and in certain diseases. Finally, data concerning the isolation and biochemical characterization of the Fc receptor molecule are presented.

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Fc‐receptor modulates the fusion between phagocytic vesicles and lysosomes in guinea pig polymorphonuclear leukocytes

Cited by 7 publications

References 6 publications

Superoxide-forming NADPH oxidase preparation of pig polymorphonuclear leucocyte

Superoxide-forming NADPH oxidase preparation of pig polymorphonuclear leucocyte

Molecular Aspects of Membrane Fusion

Fc receptor—More answers, more questions

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