Separation of the protease enzymes of Bacillus licheniformis from the fermentation medium by crossflow ultrafiltration

Takaç, Serpil; Elmas, Sema; Çalı́k, Pınar; Özdamar, Tunçer H.

doi:10.1002/1097-4660(200006)75:6<491::aid-jctb245>3.3.co;2-x

Cited by 11 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The research activities related to the enzyme separations mostly report on the successive purification steps including ultrafiltration [18][19][20][21][22]. In our previous paper, we reported the recovery conditions of protease enzymes of Bacillus licheniformis from the fermentation medium in a crossflow ultrafiltration system [23]. However, none of the studies available in the literature on serine alkaline protease (SAP) reports the interaction of the enzyme with ultrafiltration membranes under the influences of different ions.…”

Section: Introductionmentioning

confidence: 99%

Effect of ionic environments on the adsorption and diffusion characteristics of serine alkaline protease enzyme in polyethersulfone ultrafiltration membranes

Salgın

Takaç

Özdamar

2006

Journal of Colloid and Interface Science

Self Cite

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Effect of ionic environments on the adsorption and diffusion characteristics of serine alkaline protease enzyme in polyethersulfone ultrafiltration membranes

Salgın

Takaç

Özdamar

2006

Journal of Colloid and Interface Science

Self Cite

View full text Add to dashboard Cite

“…Although membrane processes have many advantages, such as low energy consumption, no phase changes, easy scale-up, the fundamental problem of industrial membrane processes when compared with classic separation techniques is membrane fouling [1][2][3]. In order to solve this problem, many researchers have used membrane zeta potential as a key parameter [4][5][6][7][8], because membrane and particle zeta potentials provide important information about the nature and magnitude of membrane fouling caused by the membrane-particle interfacial interactions.…”

Section: Introductionmentioning

confidence: 84%

Determination of correct zeta potential of polyether sulfone membranes using CLC and AGC: ionic environment effect

Salgın

Tuzlalı

2016

Desalination and Water Treatment

View full text Add to dashboard Cite

A B S T R A C TIn this study, the correct and apparent zeta potential of polyether sulfone (PES) ultrafiltration membranes at different ionic environments were determined using streaming potential measurements. The apparent zeta potential was found by calculation with classic Helmholtz-Smoluchowski (HS) and Fairbrother-Mastin (FM) equations of results obtained from clamping cell (CLC) and adjustable gap cell (AGC). Correct zeta potential was determined by calculation with a modified HS equation of obtained results from AGC. Obtained apparent zeta potential results from CLC and AGC differ from each other, and the reproducibility of results was found to be higher for measurements of AGC. Except for low pH values, apparent zeta potential that was obtained from classic HS equation with AGC could be accepted as correct zeta potential. In CLC measurements, underestimated or overestimated zeta potential values were determined in comparison with the correct zeta potential. The effect of membrane surface conductance on zeta potential decreased with increasing KCl concentration. In CLC, the deviation was observed from electrical double layer theory when KCl concentration was 0.1 M, therefore streaming potential measurements did not produce correct results at higher salt concentration. 0.001 M KCl concentration was the value at which surface conductance became crucial.

show abstract

“…Protease activity was assayed spectrophotometrically using casein as substrate [13]. One unit of enzyme activity is defined as 4 nmol tyrosine released/min per ml.…”

Section: Enzyme Assaysmentioning

confidence: 99%

Impact of Inoculation Strategy on the Progress ofCandida rugosaCultivation

Takaç

Erdem

Ünlü

2009

Artificial Cells, Blood Substitutes, and Biotechnology

Self Cite

View full text Add to dashboard Cite

Batch cultivation of Candida rugosa for the production of lipase and esterase enzymes was carried out by employing two different inoculation strategies. The effects of triolein (2, 3 and 5 g/l) and oleic acid (3 g/l) as carbon sources of the enzyme production medium on the activity, productivity, and yield of enzymes were also compared for both strategies. Inoculation of the cells into the enzyme production medium either directly or after cultivation in a pre-culture medium rich in glucose affected the activity and yield of esterase more than those of lipase for both carbon sources. In both strategies, triolein and oleic acid yielded the same lipase activity (16.67 U/ml) whereas triolein provided higher esterase activity (0.0035 U/ml). Time courses of the extracellular and intracellular lipase and esterase enzymes indicated that lipase activity was growth-associated and the cells secreted esterase into the medium after a considerable level of extracellular lipase activity was reached. The role of protease in the enzyme activities was also discussed.

show abstract

Separation of the protease enzymes of Bacillus licheniformis from the fermentation medium by crossflow ultrafiltration

Cited by 11 publications

References 0 publications

Effect of ionic environments on the adsorption and diffusion characteristics of serine alkaline protease enzyme in polyethersulfone ultrafiltration membranes

Effect of ionic environments on the adsorption and diffusion characteristics of serine alkaline protease enzyme in polyethersulfone ultrafiltration membranes

Determination of correct zeta potential of polyether sulfone membranes using CLC and AGC: ionic environment effect

Impact of Inoculation Strategy on the Progress ofCandida rugosaCultivation

Contact Info

Product

Resources

About