Separation of the Pigments Produced as a Result of Greening of Chlorogenic Acid

Abstract

Separation of the green pigments prepared by the reaction of chlorogenic acid with nitrogen-containing compounds in alkaline sodium hydrogen carbonate solution and in Menzel buffer solution, was investigated by means of Sephadex G-25, G-15 column chromatography, using distilled water or Menzel buffer solution as eluent. Using distilled water as eluent, blue-green, purple, and yellow pigments were separated from the green solution obtained by the reaction of alanine, phenylalanine, or glutamic acid with chlorogenic acid in alkaline sodium hydrogen carbonate solution. And, from the reaction mixture of phenylalanine with chlorogenic acid using Menzel buffer solution as eluent, green and yellow pigments were separated, and, using distilled water as eluent, blue-green and purple pigments were obtained. Similar results was obtained in the reaction products of phenylalanine and chlorogenic acid in Menzel buffer.

Green Pigment Formed by the Reaction of Chlorogenic Acid (or Caffeic Acid Esters) with a Primary Amino Compound during Food Processing

“…There have been several studies on the conditions under which this greening occurred (10)(11)(12)(13)(14) but the chemical structure of the green pigment is not yet known.…”