Separation of the genera in the subtribe Cassiinae (Leguminosae: Caesalpinioidae) using molecular markers

Abstract

Separation of the genera in the subtribe Cassiinae (Leguminosae: Caesalpinioidae) using molecular markers RESUMO (Separação dos gêneros na subtribo Cassiinae (Leguminosae: Caesalpinioidae) utilizando marcadores moleculares). Técnicas de Random amplified polymorphic DNA (RAPD), Inter simple sequence repeat (ISSR) e Amplified Fragment Length Polymorphism markers (AFLP) foram utilizadas para verificar a segregação do gênero Cassia L. senso lato em três diferentes gêneros, Chamaecrista Moench., Senna P. Mill. e Cassia L. senso stricto Dezoito representantes dos três táxons foram caracterizados com o uso de marcadores moleculares: 25 RAPD, seis iniciadores ("primers") ISSR e seis AFLP combinações de iniciadores, resultando na amplificação de 612, 115 e 622 bandas (loci), respectivamente. A maioria dos loci apresentou-se como polimórfico, mostrando um alto grau de diversidade genética entre os táxons estudados. O dendrograma construído com base nos dados de RAPD, ISSR e AFLP e agrupamento com procedimentos SHAN dividiu Cassia L. senso lato em três diferentes agrupamentos, chamados de Chamaecrista Moench., Senna P. Mill. e Cassia L. senso stricto Valores altos de bootstrap revelaram que todos os agrupamentos foram estáveis e robustos. Foi observado pela presente investigação que estes gêneros possuem identidade ao nível molecular, o que sustenta a elevação do genero Cassia L. senso lato para o nível de subtribo e a segregação dos três gêneros ao invés de formarem categorias infra-genéricas.Palavras-chave: AFLP, Cassia, fi logenia molecular, ISSR, RAPD Laxmikanta Acharya 1, 4 , Arup Kumar Mukherjee 2 and Pratap Chandra Panda 3 ABSTRACT(Separation of the genera in the subtribe Cassiinae (Leguminosae: Caesalpinioidae) using molecular markers). Random amplified polymorphic DNA (RAPD), Inter simple sequence repeat (ISSR) and Amplified fragment length polymorphism (AFLP) markers were used to verify the segregation of the genus Cassia L. senso lato into three distinct genera namely Chamaecrista Moench., Senna P. Mill. and Cassia L. sensostricto Eighteen representatives of the three taxa were characterized using the molecular markers. 25 RAPD, six ISSR primers and six AFLP primer combinations resulted in the amplification of 612, 115 and 622 bands (loci) respectively. Most of the loci are found to be polymorphic, showing high degrees of genetic diversity among the different taxa studied. The dendrogram constructed on the basis of the RAPD, ISSR and AFLP data using SHAN clustering, divided Cassia L. senso lato. into three different clusters as Chamaecrista Moench. Senna P. Mill. and Cassia L. senso stricto High bootstrap value revealed that all the clusters were stable and robust. It was observed from the present investigation that these genera have their identity at molecular level, which supports the elevation of the genus Cassia L. senso lato to the level of subtribe Cassiinae and segregation into three distinct genera instead of intrageneric categories. IntroductionCassia L. senso lato (Leguminosae: Caesalpinioideae) is on...

Efficiency of different PCR-based marker systems for assessment of Iris pumila genetic diversity

“…In case of I. pumila, the highest average resolving power was demonstrated by ISSR-and LP-PCR-markers (8.66 and 6.96, respectively), the rest of the techniques showed lower values: RAPD -4.98, IRAP -4.31, RGAP -3.76 (Table 2). Similarly to our results, the studies on genetic diversity in plants showed that Rp values for ISSRmarkers were generally somewhat higher than those for RAPD-markers (Behera et al 2008;Kayis et al 2010;Xavier et al 2011;Acharya et al 2011). The difference was especially considerable in studies of Hordeum vulgare cultivars, where average resolving power of ISSRand RAPD-primers made up 9.79 and 3.85, respectively (Fernandez et al 2002).…”

Intra- and interspecific karyotypic variations of the genus Senna Mill. (Fabaceae, Caesalpinioideae)

“…Species occupy an extremely wide range of habitats, varying from humid forests, dry forests, rock outcrops, dry or cold deserts to anthropized areas (Irwin & Barneby 1982;Acharya et al 2011).…”

“…Phylogenetic analyses have demonstrated that Senna is monophyletic and occupies a position near Cassia senso stricto and Chamaecrista (Marazzi et al 2006;Acharya et al 2011). Th ose three genera form the subtribe Cassiinae Irwin & Barneby, and they are morphologically distinguished in relation to characteristics of their androceu, corolla, fl oral architecture, bracteoles, and fruits.…”

Stomatal and trichome diversity in <i>Senna</i> Mill. from Bangladesh

“…The separation of Senna Mill. from Cassia L. was further established by taxonomic (Singh, 2001), phenetic (Boonkerd et al, 2005) and molecular studies (Acharya et al, 2011). In Bangladesh, Senna Mill.…”