Intra- and interspecific karyotypic variations of the genus Senna Mill. (Fabaceae, Caesalpinioideae)

Cordeiro, Joel Maciel Pereira; Félix, Leonardo P.

doi:10.1590/0102-33062017abb0274

Cited by 11 publications

(10 citation statements)

References 25 publications

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“…The chromosome number of S. floribunda (2n = 28) in this study corroborates the finding in previous reports (Rice et al 2015;Cordeiro and Felix 2018), whereas that of S. covesii has not been reported yet and was revealed as 2n = 28 in this study. Souza and Benko-Iseppon (2004) and Biondo et al (2005) reported the sizes and types of chromosomes on eleven Senna species.…”

Section: Discussionsupporting

confidence: 92%

Chromosome Karyotyping of Senna covesii and S. floribunda based on Triple?color FISH Mapping of rDNAs and Telomeric Repeats

Youn

Kim

2018

Plant Breed. Biotech.

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The genus Senna in the family Fabaceae is distributed and cultivated around the world and has been used as sources of medicine and commercial goods. Molecular cytogenetic study based on FISH technique is useful for breeding and genomic research of the species. Triple-color FISH karyotype analyses were carried out to understand the genome structure of two Senna species, S. covesii (A. Gray) H. S. Irwin & Barneby and S. floribunda (Cav.) H. S. Irwin & Barneby using 5S rDNA, 45S rDNA, and telomeric repeat probes. Three and one pairs of 45S and 5S rDNA signals were detected, respectively, in S. covesii, whereas one pair each of 45S and 5S rDNA signals, were detected in S. floribunda. Telomeric signals appeared at all chromosome termini in both species. This result provides basic cytogenetic information which will be useful for future breeding and genomic research of Senna species.

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Section: Discussionsupporting

confidence: 92%

Chromosome Karyotyping of Senna covesii and S. floribunda based on Triple?color FISH Mapping of rDNAs and Telomeric Repeats

Youn

Kim

2018

Plant Breed. Biotech.

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“…Lastly, the 2 n = 28 chromosome number is predominant in Senna and lower numbers such as 2 n = 22–26 are considered descending dysploid species (Cordeiro and Felix, 2017 ; Pellerin et al, 2019 ). In S. tora , this reduction is likely caused by a merger of two chromosomes, resulting in derivative chromosome 7, as indicated by the interstitial signal of StoTR03_178 in chromosome 7L ( Figure 3 ).…”

Section: Discussionmentioning

confidence: 99%

Chromosomal Mapping of Tandem Repeats Revealed Massive Chromosomal Rearrangements and Insights Into Senna tora Dysploidy

et al. 2021

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Tandem repeats can occupy a large portion of plant genomes and can either cause or result from chromosomal rearrangements, which are important drivers of dysploidy-mediated karyotype evolution and speciation. To understand the contribution of tandem repeats in shaping the extant Senna tora dysploid karyotype, we analyzed the composition and abundance of tandem repeats in the S. tora genome and compared the chromosomal distribution of these repeats between S. tora and a closely related euploid, Senna occidentalis. Using a read clustering algorithm, we identified the major S. tora tandem repeats and visualized their chromosomal distribution by fluorescence in situ hybridization. We identified eight independent repeats covering ~85 Mb or ~12% of the S. tora genome. The unit lengths and copy numbers had ranges of 7–5,833 bp and 325–2.89 × 106, respectively. Three short duplicated sequences were found in the 45S rDNA intergenic spacer, one of which was also detected at an extra-NOR locus. The canonical plant telomeric repeat (TTTAGGG)n was also detected as very intense signals in numerous pericentromeric and interstitial loci. StoTR05_180, which showed subtelomeric distribution in Senna occidentalis, was predominantly pericentromeric in S. tora. The unusual chromosomal distribution of tandem repeats in S. tora not only enabled easy identification of individual chromosomes but also revealed the massive chromosomal rearrangements that have likely played important roles in shaping its dysploid karyotype.

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“…The predominant chromosome number of the genus Senna is 2n = 28 (Rice et al 2015), but species with descending dysploid karyotypes of 2n = 22-26 have also been identified, such as S. tora with 2n = 26 (Cordeiro and Felix 2018;Pellerin et al 2019). To understand the dysploidization in S. tora, we examined its genome TR composition in our previous work (Waminal et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Comparative FISH analysis of Senna tora tandem repeats revealed insights into the chromosome dynamics in Senna

Waminal

Nguyen

et al. 2021

Genes Genom

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Background DNA tandem repeats (TRs) are often abundant and occupy discrete regions in eukaryotic genomes. These TRs often cause or generate chromosomal rearrangements, which, in turn, drive chromosome evolution and speciation. Tracing the chromosomal distribution of TRs could therefore provide insights into the chromosome dynamics and speciation among closely related taxa. The basic chromosome number in the genus Senna is 2n = 28, but dysploid species like Senna tora have also been observed. Objective To understand the dynamics of these TRs and their impact on S. tora dysploidization. Methods We performed a comparative fluorescence in situ hybridization (FISH) analysis among nine closely related Senna species and compared the chromosomal distribution of these repeats from a cytotaxonomic perspective by using the ITS1-5.8S-ITS2 sequence to infer phylogenetic relationships. Results Of the nine S. tora TRs, two did not show any FISH signal whereas seven TRs showed similar and contrasting patterns to other Senna species. StoTR01_86, which was localized in the pericentromeric regions in all S. tora, but not at the nucleolar organizer region (NOR) site, was colocalized at the NOR site in all species except in S. siamea. StoTR02_7_tel was mostly localized at chromosome termini, but some species had an interstitial telomeric repeat in a few chromosomes. StoTR05_180 was distributed in the subtelomeric region in most species and was highly amplified in the pericentromeric region in some species. StoTR06_159 was either absent or colocalized in the NOR site in some species, and StoIGS_463, which was localized at the NOR site in S. tora, was either absent or localized at the subtelomeric or pericentromeric regions in other species. Conclusions These data suggest that TRs play important roles in S. tora dysploidy and suggest the involvement of 45S rDNA intergenic spacers in “carrying” repeats during genome reshuffling.

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Intra- and interspecific karyotypic variations of the genus Senna Mill. (Fabaceae, Caesalpinioideae)

Cited by 11 publications

References 25 publications

Chromosome Karyotyping of Senna covesii and S. floribunda based on Triple?color FISH Mapping of rDNAs and Telomeric Repeats

Chromosome Karyotyping of Senna covesii and S. floribunda based on Triple?color FISH Mapping of rDNAs and Telomeric Repeats

Chromosomal Mapping of Tandem Repeats Revealed Massive Chromosomal Rearrangements and Insights Into Senna tora Dysploidy

Comparative FISH analysis of Senna tora tandem repeats revealed insights into the chromosome dynamics in Senna

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