Separation of surfactin from fermentation broths by acid precipitation and two-stage dead-end ultrafiltration processes

Chen, Huei-Li; Chen, Ying-Shr; Juang, Ruey‐Shin

doi:10.1016/j.memsci.2007.04.031

Cited by 65 publications

(48 citation statements)

References 29 publications

(33 reference statements)

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“…Purification was completed by chromatographic procedures (on silica gel or by reversed phase chromatography). Other methods were then proposed to purify and concentrate the lipopeptide: a two-phase extraction (Drouin and Cooper 1992), an ultrafiltration method (Mulligan and Gibbs 1990;Isa et al 2008), a solid-phase extraction (Razafindralambo et al 1993;Montastruc et al 2008), a liquid membrane extraction (Dimitrov et al 2008) or different combined methods (Chen et al 2007(Chen et al , 2008.…”

Section: Purification Of Surfactinmentioning

confidence: 99%

Surfactin and Other Lipopeptides from Bacillus spp.

Jacques

2010

Microbiology Monographs

159

167

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Section: Purification Of Surfactinmentioning

confidence: 99%

Surfactin and Other Lipopeptides from Bacillus spp.

Jacques

2010

Microbiology Monographs

159

167

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“…The CFS was purified and concentrated by Amicon Magnetically Stirred Ultrafiltration Cell (Beverly, MA, USA) employed with the two types of membranes such as YM 30 kDa (UF-I) (Millipore Cooperation, Bedford, USA) and Omega 10 kDa (UF-II) (PALL Life Sciences, USA) polyethersulfone (PES) membranes [6][7][8][9].…”

Section: Recovery Of Biosurfactant By Ultrafiltrationmentioning

confidence: 99%

“…There are several methods that have been reported for the purification of lipopeptides at small scale. These include thin layer chromatography (TLC), high performance liquid chromatography (HPLC), gel permeation chromatography (GPC), ion exchange chromatography (IEC) [4][5][6]8]. In addition to the chromatographic techniques, ultrafiltration (UF) can also be efficiently used for the small scale recovery and purification of biosurfactants in minimum possible steps [7].…”

Section: Introductionmentioning

confidence: 99%

“…In addition to the chromatographic techniques, ultrafiltration (UF) can also be efficiently used for the small scale recovery and purification of biosurfactants in minimum possible steps [7]. UF being a pressure driven membrane separation technique can be easily adapted to large scale recovery of extracellular biomolecules, like biosurfactants [6][7][8]. The critical micelle concentration (CMC) of the biosurfactant is the minimum amount required for the onset of the process of micellization.…”

Section: Introductionmentioning

confidence: 99%

“…At this and higher concentrations, the surface tension reaches a minimum value and remains constant. Above its CMC value any biosurfactant forms large micellar aggregates, which can be easily retained by the UF method with the help of different polymeric membranes with net molecular weight cut off (NMWCO) ranging from 10 to 50 kDa [6][7][8][9]. The laboratory scale recovery of surfactin using UF has been reported previously [7,9].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Single step concomitant concentration, purification and characterization of two families of lipopeptides of marine origin

Sivapathasekaran

Mukherjee

Sen

et al. 2010

Bioprocess Biosyst Eng

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The extracellular biosurfactant product secreted by a marine bacterium was concentrated and purified directly from the fermentation broth in a single step by ultrafiltration (UF) employing YM 30 kDa (UF-I) and Omega 10 kDa (UF-II) polyethersulfone membranes. The optimum operating pressure required for both membranes, UF-I and UF-II, were found to be 30 and 35 psi, respectively. The biosurfactant from the fermentation broth was recovered in higher amounts using UF-II (89%) than using UF-I (73%). An analysis of the critical micelle concentrations (CMC) of the recovered lipopeptides showed a lower CMC value of 15 mg L⁻¹ for the UF-II product, indicating higher degree of purity (83%) when compared to that of the UF-I product (78%). The ultrafiltered products were characterized using Fourier transformed infrared spectroscopy and matrix-assisted laser desorption ionization time of flight mass spectral analysis, which demonstrated the presence of two families of lipopeptides.

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In situ downstream strategies for cost‐effective bio/surfactant recovery

Najmi

Ebrahimipour

Franzetti

et al. 2018

Biotech and App Biochem

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Since 60-80% of total costs of production are usually associated with downstream collection, separation, and purification processes, it has become advantageous to investigate how to replace traditional methods with efficient and cost-effective alternative techniques for recovery and purification of biosurfactants. In the traditional techniques, large volumes of organic solvents are usually used for increasing production cost and the overall environmental burden. In addition, traditional production and separation methods typically carried out in batch cultures reduce biosurfactant yields due to product inhibition and lower biosurfactants activity as a result of interaction with the organic solvents used. However, some in situ recovery methods that allow continuous separation of bioproducts from culture broth leading to an improvement in yield production and fermentation efficiency. For biosurfactants commercialization, enhancement of product capacity of the separation methods and the rate of product removal is critical. Recently, interest in the integration of separation methods with a production step as rapid and efficient techniques has been increasing. This review focuses on the technology gains and potentials for the most common methods used in in situ product removal: foam fractionation and ultrafiltration, especially used to recover and purify two well-known biosurfactants: glycolipids (rhamnolipids) and lipopeptides (surfactins).

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Separation of surfactin from fermentation broths by acid precipitation and two-stage dead-end ultrafiltration processes

Cited by 65 publications

References 29 publications

Surfactin and Other Lipopeptides from Bacillus spp.

Surfactin and Other Lipopeptides from Bacillus spp.

Single step concomitant concentration, purification and characterization of two families of lipopeptides of marine origin

In situ downstream strategies for cost‐effective bio/surfactant recovery

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