Separation of some amino acids using ethanol-acetic acid-water: A student experiment in paper chromatography

Abstract

This experiment utilizes cylinder-shaped filter paper, capillary pipets for spotting, the dipping or rolling method of applying the reagents, and the use of multiple dipping.

Identification of amino acids in a protein hydrolysate by paper chromatography

“…The spray reagent is 0.1% solution of ninhydrin in 95% ethanol (3). The best development of colors obtains if 5 ml of collidine is added to 100 ml of the spray solution.…”