Separation of six purine bases by capillary electrophoresis with electrochemical detection

Chen, Gang; Chu, Qingcui; Zhang, Luyan; Ye, Jiannong

doi:10.1016/s0003-2670(02)00027-2

Cited by 88 publications

(41 citation statements)

References 21 publications

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“…The CE-AD system used has been described previously [50][51][52]. A 630 kV high-voltage dc power supply (Shanghai Institute of Nuclear Research, China) provided a separation voltage between the two ends of the capillary.…”

Section: Apparatusmentioning

confidence: 99%

“…The pH of the running buffer affects the zeta-potential (z), the EOF as well as the overall charge of honokiol and magnolol, which determine the migration time, peak height and the separation of the analytes [51]. The effect of the running buffer pH on the migration time of the analytes is shown in Fig.…”

Section: Effects Of the Concentration And Ph Of The Running Buffermentioning

confidence: 99%

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Carbon nanotube/poly(methyl methacrylate) composite electrode for capillary electrophoretic measurement of honokiol and magnolol in Cortex Magnoliae Officinalis

Yao

Yang

et al. 2006

Electrophoresis

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This paper describes the development and the application of a novel carbon nanotube/poly(methyl methacrylate) (CNT/PMMA) composite electrode as a sensitive amperometric detector of CE. The composite electrode was fabricated on the basis of the in situ polymerization of a mixture of CNT and prepolymerized methylmethacrylate in the microchannel of a piece of fused-silica capillary under heat. The performance of this unique system has been demonstrated by separating and detecting honokiol and magnolol in traditional Chinese medicine, Cortex Magnoliae Officinalis. Factors influencing their separation and detection processes were examined and optimized. Honokiol and magnolol were well separated within 7 min in a 40 cm long capillary at a separation voltage of 15 kV using a 50 mM borate buffer (pH 9.2). The new CNT-based CE detector offered significantly lower operating potentials, yielded substantially enhanced S/N characteristics, and exhibited resistance to surface fouling and hence enhanced stability. It demonstrated long-term stability and reproducibility with RSDs of less than 5% for the peak current (n = 9) and should also find a wide range of applications in microchip CE, flowing injection analysis, and other microfluidic analysis systems.

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Section: Apparatusmentioning

confidence: 99%

Section: Effects Of the Concentration And Ph Of The Running Buffermentioning

confidence: 99%

Carbon nanotube/poly(methyl methacrylate) composite electrode for capillary electrophoretic measurement of honokiol and magnolol in Cortex Magnoliae Officinalis

Yao

Yang

et al. 2006

Electrophoresis

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“…In addition, rapid separations are feasible with CE because high voltages can be applied to short capillaries and separation efficiency is not dependent on column length. To identify biological and pharmaceutical analysis, CE is coupled to a variety of detectors, including fluorescence, mass spectrometry, and electrochemical detection [15,16].…”

Section: Capillary Electrophoresis Methodmentioning

confidence: 99%

Study on detection methods for sorbitol

Yuan¹,

Don²

2016

Int. J. Curr. Res. Chem. Pharm. Sci

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Sorbitol has been applied as sweetener in "sugar free" food products intended for diabetics for many years. It is also applie d as starting material for Vitamin C synthesis. Furthermore, sorbitol is one of polyols commonly used in the treatment of patients with oliguric renal failure. However, more accumulation of sorbitol in tissues has been suspected as one of the causative factors of diabetic complications. Thus, the establishment of a simple and rapid method for the determination of sorbitol with high se lectivity and sensitivity is of great significance to efficient quality control of foods and pharmaceuticals and people's health. In th is article the studies of detection methods for sorbitol in recent years are reviewed.

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“…The filter of the detector was set at 0.1 Hz. The carbon disc electrode was made of a piece of 300 µm diameter graphite rod (pencil lead, Rotring, hi-Polymer, Germany) from polishing technique as descried in a previous report (Chen et al, 2002b). The side of the graphite rod was coated with epoxy (Zhejian Cixi Tiandong Adhesive Co. Ltd, Ningbo, China) for insulation.…”

Section: Methodsmentioning

confidence: 99%

Determination of active constituents inLonicera confusa DC. by capillary electrophoresis with amperometric detection

Yao

Gongyu

Chen

2006

Biomed. Chromatogr.

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A method based on capillary electrophoresis with amperometric detection has been developed for the determination of luteolin, chlorogenic acid, 3,5-dicaffeoylquinic acid and caffeic acid in the dried flower buds, leaves and stems (three medicinal parts) of Lonicera confusa DC., respectively. The effects of several important factors such as detection potential, the concentration of the running buffer, separation voltage and injection time were investigated to acquire the optimum conditions. The detection electrode was a 300 microm diameter carbon disc electrode at a working potential of + 0.90 V (vs saturated calomel electrode). The four analytes can be well separated within 10 min in a 40 cm-long fused silica capillary at a separation voltage of 12 kV in a 50 mM borate-25 mM phosphate buffer (pH 8.0). The relationship between peak current and analyte concentration was linear over about 3 orders of magnitude with detection limits (S/N = 3) ranging from 0.35 to 0.52 microM for all analytes. The proposed method has been successfully applied to the monitoring of bioactive constituents in the real plant samples with satisfactory assay results.

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Separation of six purine bases by capillary electrophoresis with electrochemical detection

Cited by 88 publications

References 21 publications

Carbon nanotube/poly(methyl methacrylate) composite electrode for capillary electrophoretic measurement of honokiol and magnolol in Cortex Magnoliae Officinalis

Carbon nanotube/poly(methyl methacrylate) composite electrode for capillary electrophoretic measurement of honokiol and magnolol in Cortex Magnoliae Officinalis

Study on detection methods for sorbitol

Determination of active constituents inLonicera confusa DC. by capillary electrophoresis with amperometric detection

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