Determination of active constituents inLonicera confusa DC. by capillary electrophoresis with amperometric detection

Abstract

A method based on capillary electrophoresis with amperometric detection has been developed for the determination of luteolin, chlorogenic acid, 3,5-dicaffeoylquinic acid and caffeic acid in the dried flower buds, leaves and stems (three medicinal parts) of Lonicera confusa DC., respectively. The effects of several important factors such as detection potential, the concentration of the running buffer, separation voltage and injection time were investigated to acquire the optimum conditions. The detection electrode was a 300 microm diameter carbon disc electrode at a working potential of + 0.90 V (vs saturated calomel electrode). The four analytes can be well separated within 10 min in a 40 cm-long fused silica capillary at a separation voltage of 12 kV in a 50 mM borate-25 mM phosphate buffer (pH 8.0). The relationship between peak current and analyte concentration was linear over about 3 orders of magnitude with detection limits (S/N = 3) ranging from 0.35 to 0.52 microM for all analytes. The proposed method has been successfully applied to the monitoring of bioactive constituents in the real plant samples with satisfactory assay results.

Simultaneous determination of aminothiols, ascorbic acid and uric acid in biological samples by capillary electrophoresis with electrochemical detection

“…The CE-ECD system used has been described previously (Chen et al, 2006a,b;Yao, 2006). A ±30 kV high-voltage dc power supply (Shanghai Institute of Nuclear Research, China) provided a separation voltage between the two ends of the capillary.…”

Binding study of Flos Lonicerae Japonicae with bovine serum albumin using centrifugal ultrafiltration and liquid chromatography

“…The result was in agreement with the previous reports (Xiang et al, 2001;Makoto et al, 2002), which showed that the caffeoyl was the active group of caffeoylquinic acid derivatives and it could increase the activity of this type of compounds. In addition, the other bound compounds such as caffeic acid, rutin, quercetin-3-O-glucoside, luteolin-3-O-glucoside, lonicerin, isomer chlorogenic acid and isomer of di-O-caffeoyl quinic acid were all proven bioactive compounds, which had been used as quality control markers of FLJ (Yao et al, 2006;Ma et al, 2005;Huang et al, 2005). Therefore, it is believed that this proposed analytical method could be applied in screening bioactive compounds in TCMs.…”

Capillary electrophoresis and microchip capillary electrophoresis with electrochemical and electrochemiluminescence detection

“…Chen's group separated and determined clenbuterol, cimaterol, and salbutamol in feed and urine [73], perphenazine and fluphenazine in tablet, spiked urine, and rabbit serum samples [74], and L-tyrosine and its metabolites in rabbit's urine [75]. Yao et al [76] monitored bioactive constituents (luteolin, chlorogenic acid, 3,5-dicaffeoylquinic acid, and caffeic acid) in real plant samples. CE -AD can also serve as an analysis tool to detect active ingredients in drug [77 -79], Chinese herbal medicines [80], and isomers [81 -83].…”