Separation of rutin nona(H-) and deca(H-) sulfonate sodium by ion-pairing reversed-phase liquid chromatography

Wang, Xiangjun; Tang, Yanhui; Yao, Tong-wei; Zeng, Su

doi:10.1016/j.chroma.2004.02.085

Cited by 7 publications

(8 citation statements)

References 14 publications

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“…Ion-pairing reversed-phase high-performance liquid chromatography (RP-HPLC) analysis of RDS The concentration of RDS was analysed by ion-pairing RP-HPLC after centrifugation of the samples at 10 000 rev min À1 for 8 min. Tetrabutyl ammonium bromide (TBA) was used as ion-pairing reagent (Wang et al 2004). The HPLC conditions were as follows: Shimadzu LC-2010C HPLC system with UV detection at 254 nm, an Aglient Zorbax Eclipse XDB-C8 column (5 m, 150 Â 4.6 mm i.d.)…”

Section: Inhibition Studiesmentioning

confidence: 99%

Transport characteristics of rutin deca (H-) sulfonate sodium across Caco-2 cell monolayers

Zeng

2005

Journal of Pharmacy and Pharmacology

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The aim of this study was to explore potential transport mechanisms of rutin deca (H‐) sulfonate sodium (RDS) across Caco‐2 cell monolayers. As an in‐vitro model of human intestinal epithelial membrane, Caco‐2 cells were utilized to evaluate the transepithelial transport characteristics of this hydrophilic macromolecular compound. Bi‐directional transport study of RDS demonstrated that the apparent permeability (Papp) in the secretory direction was 1.4∼4.5‐fold greater than the corresponding absorptive Papp at concentrations in the range 50.0∼2000 μm. The transport of RDS was shown to be concentration, temperature and pH dependent. In the presence of ciclosporin and verapamil, potent inhibitors of P‐glycoprotein (P‐gp)/MRP2, the absorptive transport was enhanced and secretory efflux was diminished. RDS significantly reduced the efflux ratio of the P‐gp substrate rhodamine‐123 in a fashion indicative of P‐gp activity suppression, while rhodamine‐123 competitively inhibited the polarized transport of the compound. In conclusion, the results indicated that RDS was likely a substrate of P‐gp. Several efflux transporters, including P‐gp, participated in the absorption and efflux of RDS and they might play significant roles in limiting the oral absorption of the compound. These observations offered important information for the pharmacokinetics of RDS.

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Section: Inhibition Studiesmentioning

confidence: 99%

Transport characteristics of rutin deca (H-) sulfonate sodium across Caco-2 cell monolayers

Zeng

2005

Journal of Pharmacy and Pharmacology

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“…A method for determining of RDS in raw materials has been reported by us earlier using ion-pairing high-performance liquid chromatography (IP-HPLC) [13]. As the concentrations of RDS prepared for analysis from raw materials are much higher than those in plasma, but the method cannot be used directly to measure plasma levels of RDS, due to inadequate sensitivity or interference by endogenous components in plasma.…”

Section: Introductionmentioning

confidence: 98%

Determination of rutin deca(H-) sulfate sodium in rat plasma using ion-pairing liquid chromatography after ion-pairing solid-phase extraction

Wang

Jin

Ying

et al. 2006

Journal of Chromatography B

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“…In particular, they obtained rutin persulfate (58) (scheme 21.16) in a shorter reaction time and in quantitative yield (99%) with high purity (>95%) compared with the previously described method [39], starting from unprotected rutin (57).…”

Section: Sulfationmentioning

confidence: 99%

A Journey into Recent Microwave‐Assisted Carbohydrate Chemistry

Corsaro

Pistarà

Chiacchio

et al. 2012

Microwaves in Organic Synthesis

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Separation of rutin nona(H-) and deca(H-) sulfonate sodium by ion-pairing reversed-phase liquid chromatography

Cited by 7 publications

References 14 publications

Transport characteristics of rutin deca (H-) sulfonate sodium across Caco-2 cell monolayers

Transport characteristics of rutin deca (H-) sulfonate sodium across Caco-2 cell monolayers

Determination of rutin deca(H-) sulfate sodium in rat plasma using ion-pairing liquid chromatography after ion-pairing solid-phase extraction

A Journey into Recent Microwave‐Assisted Carbohydrate Chemistry

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