Separation of protein mixtures by bioran® porous glass membranes

Schnabel, R.; Langer, P.; Breitenbach, Stefan

doi:10.1016/0376-7388(88)80006-1

Cited by 40 publications

(12 citation statements)

References 1 publication

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“…Peptide hydrodynamic radius (rs) was estimated from MW according to Schnabel et al (1988) (Table I). Charge and isoelectric point of each peptide were calculated from dissociated constants and ionic groups according to Skoog and Wichman (1989) (Table I).…”

Section: Identification Of Peptides and Selection Of Tracersmentioning

confidence: 99%

Ionic interactions in nanofiltration of β casein peptides

Garem

Daufin

Maubois

et al. 1998

Biotechnol. Bioeng.

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Section: Identification Of Peptides and Selection Of Tracersmentioning

confidence: 99%

Ionic interactions in nanofiltration of β casein peptides

Garem

Daufin

Maubois

et al. 1998

Biotechnol. Bioeng.

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“…Thus, the deposited particle layer might increase rejection by a sieving effect or depth filtration. Moreover, as discussed above, the fouling layer on membrane M1 consisted only of macromolecular proteins (250 kDa ≈ 11 nm using d = 0.007 M 0.4 ) . Thus, as introduced by Kuberkar and Davis, already deposited particle might sieve the macromolecular proteins or delay their transport to the membrane surface, eventually lowering pore blockage and increasing DOC rejection…”

Section: Resultsmentioning

confidence: 96%

Advanced tools for fluid and fouling layer characterization applied to the description of membrane fouling phenomena for particle‐organic matter mixtures

et al. 2014

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Fouling by the formation of a complex cake remains a major hindrance of membrane bioreactors. One approach to fouling mitigation is to add particles within the biofluid in order to improve sludge filterability. Despite a number of studies dealing with this topic, fouling mechanisms are still not clear. This study reports the use of an original methodology for better fouling understanding and mitigation. This original approach is based on in situ characterization of cake local structural properties together with a detailed fluid characterization using size exclusion chromatography and the monitoring of global filtration performances. In order to obtain comprehensive elements, the impact of particle addition within cakes having markedly different behaviours (slightly and highly compressible cake, different specific resistance) was investigated considering different biofluids and membranes. The results demonstrated that a potential beneficial effect of particles on filtration performance depended on the cake properties in the absence of particles. It was found that particle addition could only improve biofluid filterability when the fouling layer was highly compressible and consisted mainly of macromolecular protein. In addition, in situ characterization revealed that particles within the fouling layer strongly influenced organic matter deposition at the membrane. It also showed that the effect of particles on fouling mitigation depended on the environment surrounding them within the fouling layer

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“…This opens up the possibility of using steam sterilization for the nanoporous carbon membranes and modules, which could significantly reduce the bioburden in downstream processing operations. To verify the feasibility of performing steam sterilization, an untreated nanoporous carbon membrane was exposed to stream at 120°C for 30 min at a pressure of 1 atm (1,17). The performance characteristics of the membrane after exposure to steam are summarized in Table 2.…”

Section: Results and Analysismentioning

confidence: 99%

Performance Characteristics of Nanoporous Carbon Membranes for Protein Ultrafiltration

2007

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Nanoporous carbon membranes could be very attractive for applications of ultrafiltration in the biotechnology industry because of their greater mechanical strength and longer membrane life. The objective of this study was to obtain quantitative data on the performance characteristics of nanoporous carbon membranes formed within a stainless steel support that was first modified by deposition of silica particles within the macroporous support. The nanoporous carbon membrane effectively removed small solutes from a protein solution using diafiltration, with performance comparable to that of commercial polymeric membranes. Protein fouling was evident, although the nanoporous carbon membranes were easily regenerated; cleaning with 0.5 N NaOH at 50 degrees C completely restored the water permeability for multiple cycles. The nanoporous carbon membranes were also compatible with steam sterilization. Significant increases in process flux could be obtained using periodic back-pulsing, with no evidence of any structural alterations in the membrane. These results clearly demonstrate the potential benefits and opportunities for using nanoporous carbon membranes for protein ultrafiltration.

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Separation of protein mixtures by bioran® porous glass membranes

Cited by 40 publications

References 1 publication

Ionic interactions in nanofiltration of β casein peptides

Ionic interactions in nanofiltration of β casein peptides

Advanced tools for fluid and fouling layer characterization applied to the description of membrane fouling phenomena for particle‐organic matter mixtures

Performance Characteristics of Nanoporous Carbon Membranes for Protein Ultrafiltration

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