Ionic interactions in nanofiltration of β casein peptides

Antioxidant properties of tofu whey concentrate by freeze concentration and nanofiltration processes

“…According Speroni et al (2010), during the processing of tofu the isoflavones may be involved in different kinds of interactions with proteins because of their diverse polarity and hydrophobicity characteristics, as well as their ability to form hydrogen bonds. On the other hand, Garem et al (1998) noted that the presence of high molar mass compounds, such as proteins and …”

Membrane fractionation of a β-lactoglobulin tryptic digest: Effect of the pH

“…Membrane separation using tight ultrafiltration (UF) and nanofiltration (NF) approaches have been used to fractionate protein hydrolysates (Garem et al, 1998;Pouliot et al, 1999;Groleau et al, 2004;Lapointe et al, 2005). Publications on this area highlight the important role of membrane-peptide electrostatic interactions in the differential separation of peptides with similar molecular weight (MW) but with different net charge.…”

Advancements in the Fractionation of Milk Biopeptides by Means of Membrane Processes

“…Another subsequent work reported the interesting potential of NF membranes for separating peptides in the range of 0.3-1 kDa [68]. Specific conditions of ionic strength and especially pH promoted the separation of peptides because the membrane and peptides showed amphoteric properties.…”

“…Several authors have demonstrated the preponderance of a selectivity based on electrostatic interactions at low ionic strength values [16,68,85]. The fact that electrostatic interactions membrane-peptide lose significance at high ionic strength values results in a decrease of the double selectivity size/charge in processes involving NF membranes.…”

“…Studies involving peptides transmission or retention don´t usually take into account the polarization and fouling phenomena but it has been demonstrated that these phenomena are crucial in the case of protein hydrolysates, especially at acid pH values [16,68]. Complex peptide mixtures contain peptides, which with different physicochemical characteristics (pI, hydrophobicity, charge) promote the creation of strong interactions with filtration membranes [92][93].…”