Membrane fractionation of a β-lactoglobulin tryptic digest: Effect of the pH

Fernández, Ayoa; Suárez, Andrés Santiago Suárez; Zhu, Yishen; Fitzgerald, Richard J.; Riera, Francisco A.

doi:10.1016/j.jfoodeng.2012.07.030

Cited by 23 publications

(28 citation statements)

References 23 publications

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“…El pH de la corriente de alimentación es uno de los factores más importantes en el incremento del coeficiente de ensuciamiento (CE) y en el desarrollo del fenómeno de polarización por concentración (CP); los cuales son los principales responsables de la reducción del flujo de permeado [27]. Así la tendencia al ensuciamiento de la membrana se reduce cuando el pH de la solución es básico gracias a que ocurre una repulsión electrostática entre los péptidos, los cuales tienen una carga eléctrica negativa y un punto isoeléctrico bajo [32].…”

Section: ) Phunclassified

Tecnología de membranas: Ultrafiltración

Solís¹,

Vélez²,

Ramírez-Navas³

2017

Entre cienc. ing.

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Resumen-Las Tecnologías de Membranas TM tienen gran incidencia en el desarrollo de nuevos y mejores productos, en la conservación del medio ambiente, en la industria de pinturas y la medicina, entre otros. En la industria de los alimentos las TM se aplican en diversas áreas, por ejemplo, en la desalinización de agua de mar, en el tratamiento de aguas residuales y en la clarifi cación de jugos. En el caso de los lácteos, se la ha empleado en la producción de nuevos derivados, como es el caso de las proteínas del lactosuero o la lactosa. En la presente revisión, se estudió el uso de la Ultrafi ltración UF. Se hace un especial énfasis en la industria láctea, donde se esboza el creciente auge de las TM, gracias a que permite la retención y separación de partículas, a que es amigable con el medio ambiente y a que permite el desarrollo de nuevos alimentos. Finalmente es indispensable continuar buscando alternativas para controlar la colmatación de las membranas, logrando extender la vida útil de estos materiales, ya que es el fenómeno que más las afecta. Palabras clave-Tecno logías de membrana, ultrafi ltración, separación, proteínas del lactosuero.

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Section: ) Phunclassified

Tecnología de membranas: Ultrafiltración

Solís¹,

Vélez²,

Ramírez-Navas³

2017

Entre cienc. ing.

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“…The hydrolysis was carried out under the optimum temperature and pH conditions for enzyme activity, i.e., 37 C and 8.0, respectively, and at a final enzyme/ substrate ratio (E/S) of 1/250. A 2.5 L solution containing 15 g L À1 of the substrate was used for hydrolysate preparation as previously described by Fernández Suárez, Zhu, FitzGerald, and Riera (2013) with few modifications. The TH sample was taken after 24 h of reaction.…”

Section: Preparation Of Different Bovine B-lg Fractionsmentioning

confidence: 99%

“…Each membrane has a filtration area of 0.1 m 2 . The fractionation experiments were carried out with no salt addition and at pH 8.0 according to Fernández et al (2013), Fernández and Riera (2013a). Transmembrane pressure and the temperature of the hydrolysate were set to 7.5 Â 10 4 Pa and 37 C, respectively.…”

Section: Preparation Of Different Bovine B-lg Fractionsmentioning

confidence: 99%

“…49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 INDA3550_proof ■ 25 July 2013 ■ 2/9 ö 2 ö 2 freeze-dried samples were analysed using a reversed freeze-dried samples were analysed using a reversed phase-high phase-high (BioW (BioW ittaker ittaker (BioW (BioW ittaker) ittaker) to the assays and it as always complete. Then, peptide soluet al (2013), Fernández and Riera (2013a).…”

Section: Pbmc Proliferation Assaymentioning

confidence: 99%

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Immunomodulatory activities of whey β-lactoglobulin tryptic-digested fractions

Rodríguez‐Carrio

Fernández

Riera

2014

International Dairy Journal

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“…There has been considerable interest in membrane filtration of protein hydrolysates during the last decade. However, the stress has been put in, for example, obtaining products with specific characteristics 14 or filtration parameters optimization 15 ; while little attention has been given to attempt to explain the mechanisms underlying the separation, except for 16 , 17 .…”

Section: Introductionmentioning

confidence: 99%

Utilization of blood by-products: An in silico and experimental combined study for BSA usage

Arrutia

Fernández

Menéndez

et al. 2017

Sci Rep

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In order to exploit industrial discards, protein enzymatic hydrolysis is a currently popular methodology for obtaining bioactive peptides. However, once released, most promising peptides have to be selected from the mixture. In this work, the suitability of pepsin (EC 3.4.23.1) to hydrolyse serum albumin in order to obtain bioactive peptides was assessed. Then, a suitable process to obtain best separation of bioactive peptides was evaluated, using polyethersulfone membranes at different pH values. Serum albumin was easily hydrolysed by pepsin, reaching a DH value of the 65.64 ± 1.57% of the maximum possible. A 23.25% of the identified peptides possessed high bioactivity scores (greater than 0.5), and one of them had reported bioactivity (LLL). Charge mechanisms always predominated over the sieve effect, and best transmission was accomplished at pH values close to the peptides isoelectric points. Basic and neutral peptides with the highest scores were always the most transmitted. Membrane material had greater influence than NMWCO in determining peptide transmission. In order to obtain purified fractions rich in peptides with high bioactivity scores from serum albumin, polyethersulfone membranes (applicable to industrial scale) of 5 kDa MWCO should be used at basic pH values after pepsin digestion.

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Membrane fractionation of a β-lactoglobulin tryptic digest: Effect of the pH

Cited by 23 publications

References 23 publications

Tecnología de membranas: Ultrafiltración

Tecnología de membranas: Ultrafiltración

Immunomodulatory activities of whey β-lactoglobulin tryptic-digested fractions

Utilization of blood by-products: An in silico and experimental combined study for BSA usage

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