Separation of principal component dihydromyricetin from Ampelopsis grossedentata by high‐speed counter‐current chromatography and its interaction with corn starch

Abstract

Ampelopsis grossedentata (AG) is an industrial crop in the grape family, which has been used as a dual-purpose plant for medicine and tea with high medicinal values. However, little is reported on the separation technology of active components from AG and processing technology of AG products. High-speed counter-current chromatography (HSCCC) was applied to separate the principal component dihydromyricetin (DMY) from AG. DMY is added to starch-based products to improve food quality. The interaction between corn starch (CS) and DMY was investigated to predict and control the structure and function of starchbased foods. Results show that DMY with 97.13% purity was successfully obtained by HSCCC using a solvent system composed of light petroleum-ethyl acetatemethanol-water-trichloroacetic acid (1:3:1:3:0.01, v/v/v/v/v). Fourier-transform infrared spectroscopy (FT-IR) exhibits that the interactions between CS and DMY included hydrogen bond and noncovalent bond. X-ray diffraction (XRD) shows that DMY could increase the relative crystallinity of CS. Low-field nuclear magnetic resonance results (LF-NMR) imply that DMY decreased the spin relaxation time (T 2 ) and inhibited the mobility of free water. Atomic force microscopy (AFM) results suggest that DMY changed the surface morphology of CS through hydrogen bond interaction. Moreover, the results of confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) indicate that DMY could enlarge the pores and change the microstructure of CS-DMY complexes.The findings promote the development of industrial CS-based products and utilization of corn crop.

Liquid–liquid chromatography as a promising technology in the separation of food compounds

“…In another study, dihydromyricetin was isolated from the Asian tea herb Ampelopsis grossedentata (Vitaceae) with LLC. The purified polyphenol, obtained with an enhanced purity and in a shorter isolation time compared to the previously used column chromatography processes on polyamide and macroporous resins, was proven to improve corn starch quality (Xue et al, 2022). Those studies indicate the promising future role of LLC in food and dietary supplement enrichment.…”

A model for continuous sample feed and separation with counter‐current chromatography based on elution‐extrusion mode and its application

“…In addition, the solute can fill the entire volume of the chromatographic column, so CCC has higher sample recovery and sample loading than traditional chromatography techniques. CCC has been widely used in natural products [4][5][6]. In the early stage, some research on continuous CCC technology used multiple resin columns in combination and then used buffer as the mobile phase, similar to a technique that simulates fluid bed separation.…”