Separation of porphyrin-based photosensitizer isomers by laser-induced fluorescence capillary electrophoresis

Peng, Xuejun; Sternberg, Ethan D.; Dolphin, David

doi:10.1002/elps.200500183

Cited by 10 publications

(8 citation statements)

References 43 publications

(34 reference statements)

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“…Since only a few chiral compounds possess native fluorescence, derivatization procedures are usually required to detect other nonfluorescent analytes. LIF was used for the chiral determination of photosensitizers [32], drugs in human plasma [33], and anticancer agents in drugs [34] achieving LODs around 10 28 M. However, the main application of this detection system in CE was the analysis of amino acid enantiomers in standards [35], foods [36], and biological samples [28,[37][38][39][40][41][42]. In all cases, the methods involved precapillary chiral derivatization of the amino acids with different derivatizing reagents, FITC [35,36], 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) [37], CBI [28,[38][39][40][41], or fluorescamin (FA) [42].…”

Section: On-column Detection In Chiral Analysis By Cementioning

confidence: 99%

Sensitive chiral analysis by CE: An update

et al. 2007

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Sensitive chiral analysis by CE: An updateA general view of the different strategies used in the last years to enhance the detection sensitivity in chiral analysis by CE is provided in this article. With this purpose and in order to update the previous review by García-Ruiz et al., the articles appeared on this subject from January 2005 to March 2007 are considered. Three were the main strategies employed to increase the detection sensitivity in chiral analysis by CE: (i) the use of off-line sample treatment techniques, (ii) the employment of in-capillary preconcentration techniques based on electrophoretic principles, and (iii) the use of alternative detection systems to the widely employed on-column UV-Vis absorption detection. Combinations of two or three of the above-mentioned strategies gave rise to adequate concentration detection limits up to 10 210 M enabling enantiomer analysis in a variety of real samples including complex biological matrices.

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Section: On-column Detection In Chiral Analysis By Cementioning

confidence: 99%

Sensitive chiral analysis by CE: An update

et al. 2007

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“…The developed method was able to reproducibly separate metyrosine with a resolution of 2.1 and an LOD of 1.0 mg/mL. Enantiomers of a diethyleneglycol diester benzoporphyrin derivative were successfully separated using sodium cholate micelles in a borate buffer with 30% ACN (to prevent analyte aggregation) [45]. Better resolution was obtained when the surfactant concentration was increased to 150 mM; still higher concentrations had a negative effect on the separation and caused precipitation.…”

Section: Micellesmentioning

confidence: 99%

Review of aqueous chiral electrokinetic chromatography (EKC) with an emphasis on chiral microemulsion EKC

Kahle

Foley

2007

Electrophoresis

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Review of aqueous chiral electrokinetic chromatography (EKC) with an emphasis on chiral microemulsion EKCThe separation of enantiomers using electrokinetic chromatography (EKC) with chiral microemulsions is comprehensively reviewed through December 1, 2006. Aqueous chiral EKC separations based on other pseudostationary phases such as micelles and vesicles or on other chiral selectors such as CDs, crown ethers, glycopeptides, ligand exchange moeities are also reviewed from both mechanistic and applications perspective for the period of

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“…Isomeric separation of sulpho-PCs has brought about many developments. Most of the separation techniques available involve HPLC [10,21,23,28], although separation by capillary electrophoresis (CE) [22,25,29] has also been reported. Compared to CE, HPLC is more widely used for its reproducibility and wide scope of application from analysis to preparation.…”

Section: Introductionmentioning

confidence: 99%

Isomeric separation and identification of tetra-, tri-, and di-β-sulphonic phthalocyanine zinc complexes

Zhou

Yao

et al. 2011

J. Porphyrins Phthalocyanines

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The synthesis, isomeric separation, and identification of β-sulphonic phthalocyanine zinc complexes were reported. While the sulphonic phthalocyanines have been studied extensively, the development of separation technology may enable deeper insights into their isomeric constitution. An ion-pair reversed-phase high performance liquid chromatography (IP-RP-HPLC) method was developed to separate the sulphonic phthalocyanine isomers. The results showed that the product of the condensation reaction is a mixture of all possible isomers with statistical distribution. Several isomers were obtained and structural determination was undertaken by NMR. Based on the IP-RP-HPLC elution sequence of these well-identified isomers, a relationship between the structure and efficiency was deduced: closely spaced intervals of sulphonic groups lead to higher hydrophobicity and shorter retention times on HPLC. Based on this relationship, each HPLC peak was assigned to the corresponding isomeric structure.

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Separation of porphyrin-based photosensitizer isomers by laser-induced fluorescence capillary electrophoresis

Cited by 10 publications

References 43 publications

Sensitive chiral analysis by CE: An update

Sensitive chiral analysis by CE: An update

Review of aqueous chiral electrokinetic chromatography (EKC) with an emphasis on chiral microemulsion EKC

Isomeric separation and identification of tetra-, tri-, and di-β-sulphonic phthalocyanine zinc complexes

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