Separation of multiphosphorylated cyclopeptides and their positional isomers by hydrophilic interaction liquid chromatography (HILIC) coupled to electrospray ionization mass spectrometry (ESI-MS)

Zeid, Lana Abou; Pell, Albert; Tytus, Théo; Delangle, Pascale; Bresson, Carole

doi:10.1016/j.jchromb.2021.122792

Cited by 4 publications

(1 citation statement)

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“…The primary factors affecting the formation of charged ions in ESI sources are the capillary voltage, high temperature, and nitrogen. The ionization degree of the sample can be changed by adjusting the voltage, temperature, and gas flow in the ESI source [ 31 , 32 ]. Therefore, the parameters, such as the capillary voltage, desolvation temperature, desolvation gas flow, cone gas flow, and nebulizer gas flow in the ESI source within a range, were adjusted.…”

Section: Resultsmentioning

confidence: 99%

Accurate Determination of the Degree of Deacetylation of Chitosan Using UPLC–MS/MS

Xue

Wang

Yang

et al. 2022

IJMS

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The mole fraction of deacetylated monomeric units in chitosan (CS) molecules is referred to as CS’s degree of deacetylation (DD). In this study, 35 characteristic ions of CS were detected using liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS/MS). The relative response intensity of 35 characteristic ion pairs using a single charge in nine CS samples with varying DDs was analyzed using 30 analytical methods. There was a good linear relationship between the relative response intensity of the characteristic ion pairs determined using ultrahigh performance (UP) LC–MS/MS and the DD of CS. The UPLC–MS/MS method for determining the DD of CS was unaffected by the sample concentration. The detection instrument has a wide range of application parameters with different voltages, high temperatures, and gas flow conditions. This study established a detection method for the DD of CS with high sensitivity, fast analysis, accuracy, stability, and durability.

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Section: Resultsmentioning

confidence: 99%