Determination of the affinity of biomimetic peptides for uranium through the simultaneous coupling of HILIC to ESI-MS and ICP-MS

Atomic spectrometry update: review of advances in elemental speciation

“…These results are in agreement with those previously obtained by other spectroscopic techniques, which were used to validate the method. In the second paper 143 the developed molecular and inorganic MS detection system was used to determine the effect of competing divalent metals including, Ca, Cu and Zn, on the binding of the uranyl ion UO 2 2+ to a synthetically prepared model tetra-phosphorylated peptide. Because of the isobaric interferences on the main Ca isotope Sr was used as a substitute.…”

“…The binding and stability of U within biological systems and with biomolecules is of interest from a biogeochemical and toxicological perspective and this area has been investigated and described in two recent papers. An analytical strategy developed 142 to investigate the affinity of synthetically prepared, termed biomimetic, peptides for U was based on the simultaneous coupling of HILIC with ES-MS and ICP-MS. In this instrumental set-up the peptide complexes formed with UO 2 2+ were structurally identified using negative ion ES-MS/MS, which showed that doubly charged [UO 2 (peptide)] 2− complexes with 1 : 1 stoichiometry were formed.…”