Separation of monomeric and dimeric phenolic compounds in lignosulphonate lignin on different stationary phases using ultrahigh-performance supercritical fluid chromatography

Prothmann, Jens; Palmer, Simon; Turner, Charlotta; Sandahl, Margareta

doi:10.1016/j.chroma.2021.462408

Cited by 8 publications

(10 citation statements)

References 23 publications

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“…The underlying reason for the better selectivity is suggested to be the presence of additional π–π interactions of the tested analytes on the aminoanthracene group. This is further supported by 2-PIC being the second best performing stationary phase, similarly to what has been found previously by our research group . On the other hand, the DEA column performed the worst in terms of peak resolution, leaving three peak pairs with very poor resolution ( R s < 1) in the DAD chromatogram and two peak pairs completely coeluting in the CAD chromatogram.…”

Section: Resultssupporting

confidence: 89%

“…This is further supported by 2-PIC being the second best performing stationary phase, similarly to what has been found previously by our research group. 25 On the other hand, the DEA column performed the worst in terms of peak resolution, leaving three peak pairs with very poor resolution ( R s < 1) in the DAD chromatogram and two peak pairs completely coeluting in the CAD chromatogram. It can also be observed from Figure 4 that fewer analytes were detected by the charged aerosol detector since monomers without an additional alcohol function are too volatile for CAD.…”

Section: Resultsmentioning

confidence: 99%

“…Obtaining baseline separation of peaks before the charged aerosol detector is of utmost importance since this detector is assumed to be nonselective for compounds with low volatility . In our research group, several studies have been conducted on lignin analysis by SFC , with thorough optimization of mobile and stationary phases; however, it was believed that column screening was necessary to find the stationary phase with optimal selectivity for this particular type of sample. On the other hand, the type of modifier, makeup solvent, and additives were not considered in our developmental work, but the choice was based on literature information …”

Section: Resultsmentioning

confidence: 99%

“…While CAD has been used for a wide range of applications with LC separation, , its coupling to supercritical fluid chromatography (SFC) is still yet to be more thoroughly explored. SFC/CAD offers rapid and highly selective separation − with hypothetically universal detection as demonstrated by Takeda et al for lipid analysis . However, to our knowledge, no such studies for quantification of lignin-derived compounds in general or dimers from a RCF in particular have been published.…”

Section: Introductionmentioning

confidence: 99%

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Single-Standard Quantification Strategy for Lignin Dimers by Supercritical Fluid Chromatography with Charged Aerosol Detection

et al. 2022

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The increased interest in utilizing lignin as a feedstock to produce various aromatic compounds requires advanced chemical analysis methods to provide qualitative and quantitative characterization of lignin samples along different technology streamlines. However, due to the lack of commercially available chemical standards, routine quantification of industrially relevant lignin oligomers in complex lignin samples remains a challenge. This study presents a novel method for universal quantification of lignin dimers based on supercritical fluid chromatography with charged aerosol detection (CAD). A series of lignin-derived dimeric compounds that have been reported from reductive catalytic fractionation (RCF) were synthesized and used as standards. The applicability of using linear regression instead of quadratic calibration curves was evaluated over a concentration range of 15–125 mg/L, demonstrating that the former calibration method is as appropriate as the latter. The response factors of lignin dimeric compounds were compared to assess the uniformity of the CAD signal, revealing that the CAD response for the tested lignin dimers did not differ substantially. It was also found that the response factors were not dependent on the number of methoxy groups or linkage motifs, ultimately enabling the use of only one calibrant for these compounds. The importance of chromatographic peak resolution in CAD was stressed, and the use of a digital peak sharpening technique was adopted and applied to address this challenge. The developed method was verified and used for the quantification of lignin dimers in an oil obtained by a RCF of birch sawdust.

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Single-Standard Quantification Strategy for Lignin Dimers by Supercritical Fluid Chromatography with Charged Aerosol Detection

et al. 2022

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“…Beberapa binder lokal seperti tepung jagung, tepung tapioka dan tepung sagu memiliki kandungan protein yang relatif kecil yaitu berturut-turut sebesar 3,5%, 1,1%, dan 0,2% [11]. Sedangkan pada binder LignoBond DD yang merupakan pengikat sintesis tidak memiliki kandungan protein hanya berupa sulfur, asam, natrium, magnesium, amonium atau kalsium sebagai counter ion [14]. Pengikat sintetis diproduksi secara artifisial dan tidak selalu meningkatkan nilai gizi, sebagai contoh yaitu Urea-formaldehida, Na atau Ca bentonit [15].…”

Section: Protein Kasar Pelet Indigofera Zollingerianaunclassified

Kualitas Kimia Pelet Hijauan Indigofera zollingeriana dengan Menggunakan Berbagai Jenis Binder Pelet

Yunita

Bain²,

Kurniawan³

et al. 2022

JIPHO

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Abstrak. Penelitian ini bertujuan untuk mengevaluasi kualitas kimia pelet berbahan Indigofera zollingeriana dengan menggunakan berbagai jenis binder yang berbeda. Rancangan penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 4 ulangan, sehingga terdapat 16 unit satuan percobaan. Masing-masing perlakuan menggunakan tepung Indigofera zollingeriana sebanyak 4 kg. Perlakuan yang digunakan dalam penelitian ini adalah P1= Tepung hijauan Indigofera zollingeriana + 1% binder komersial (LignoBond DD), P2 = Tepung hijauan Indigofera zollingeriana + 1% tepung sagu, P3 = Tepung hijauan Indigofera zollingeriana + 1% tepung jagung, P4 = Tepung hijauan Indigofera zollingeriana + 1% tepung tapioka. Variabel penelitian yang diamati adalah uji kimia pelet yang meliputi analisa kadar protein kasar (PK), bahan kering (BK), serta bahan organik (BO) pelet. Berdasarkan hasil penelitian, penambahan berbagai jenis binder pelet memberikan pengaruh yang nyata (P<0,05) pada uji bahan kering pelet dan bahan organik pelet, serta memberikan pengaruh yang tidak nyata (P>0,05) pada protein kasar pelet. Kesimpulan penelitian ini yaitu kualitas kimia pelet yang baik terdapat pada P2 dengan penambahan binder tepung sagu.Kata Kunci : Indigofera zollingeriana, LignoBond DD, Tepung Sagu, Tepung Jagung, Tepung Tapioka.

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Preparation of polyvinyl pyrrolidone stationary phase and its application in separation and analysis of flavonoids

Chen

et al. 2023

Separation Science Plus

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Polyvinylpyrrolidone (PVP) is known as a polymer with special adsorption properties for polyphenolic compounds. In this paper, chemical‐bonded stationary phase (PVP@Silica) was prepared and applied to the separation of flavonoids. System constants determined by linear solvation energy relationships with methanol–water mixtures as the mobile show that the column can provide stronger hydrogen bonding and π–π interaction than C18 column. Under reversed‐phase conditions, traditional flavonoids have much longer retention on PVP@Silica than on C18 column with methanol–water mobile phase due to multimodal retention mechanism, including hydrogen bonding and π–π interaction. In addition, a U‐shaped retention curve was observed in acetonitrile–water mobile phase because of the enhanced hydrogen bonding under the high proportion of acetonitrile. The selectivity to polyhydroxy structure gives the stationary phase unique separation ability for flavonoids. The separation orthogonality was further investigated by a sample set containing 33 flavonoids with different substitution structures. The (RP‐PVP@Silica)‐(RP‐C18) system exhibited 60% orthogonality metric (OM) for these flavonoids with methanol–water mobile phase. A high OM of 63.5% was achieved in (RP‐PVP@Silica)‐(HILIC‐PVP@Silica) system. Finally, PVP@Silica was applied to the purification of total flavonoids in Ginkgo biloba extract (GBE). The offline (RP‐PVP@Silica)‐(RP‐C18) two‐dimensional separation system was used for the analysis of flavonoids in GBE.

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Separation of monomeric and dimeric phenolic compounds in lignosulphonate lignin on different stationary phases using ultrahigh-performance supercritical fluid chromatography

Cited by 8 publications

References 23 publications

Single-Standard Quantification Strategy for Lignin Dimers by Supercritical Fluid Chromatography with Charged Aerosol Detection

Single-Standard Quantification Strategy for Lignin Dimers by Supercritical Fluid Chromatography with Charged Aerosol Detection

Kualitas Kimia Pelet Hijauan Indigofera zollingeriana dengan Menggunakan Berbagai Jenis Binder Pelet

Preparation of polyvinyl pyrrolidone stationary phase and its application in separation and analysis of flavonoids

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