Separation of <i>p</i>-aminobenzoic acid derivatives by paper chromatography

Kelemen, E.; Tanos, B; Halmagyi, D

doi:10.1042/bj0470138

Cited by 11 publications

(3 citation statements)

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“…Chloroacetic acid and a-chloropropionic acid have been separated on paper with use of indi~ators,~~3 and zones containing keto-acids have been made visible by conversion into the semicarbazones and viewing under ultraviolet light; 274 the method has been applied to small amounts of hydroxy-benzoic acids and amides 275 and to p-aminobenzoic acid derivatives. 276 The behaviour of numerous phenolic compounds has been studied277 and applied to analysis of tea catechins 278 and wood extracts; 279 in a very elegant analysis of certain flavonol 3-glycosides, the pigment zones were located under ultra-violet light, leached from the paper with aluminium chloride, and the ultra-violet absorption intensities of the flavonoidaluminium complexes were measured on a Beckman spectrometer. 280 Halogenated aliphatic and aromatic hydrocarbons, and n-octanol have been held as the static phase on acetylated paper, for the separation of the dinitrophenylhydrazones of the carbonyl fission products of sugars.…”

Section: Methods Involvingmentioning

confidence: 99%

Analytical chemistry

Cropper¹,

Wilson²,

Stagg³

1950

Annu. Rep. Prog. Chem.

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Section: Methods Involvingmentioning

confidence: 99%

Analytical chemistry

Cropper¹,

Wilson²,

Stagg³

1950

Annu. Rep. Prog. Chem.

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“…Chromatograms were also done on Whatman No. 1 and Scideicher and Schueil strips using a solution of butanol saturated with water (11). In this system the amine showed an R I ~ 0.71 on the Whatman ~o.…”

Section: E X P E R~n T a Lmentioning

confidence: 99%

The Isolation and Identification of Para-Aminobenzoic Acid Produced by Staphylococci Resistant to Sulfonamide

Leskowitz

Fox

Raymond

1952

Journal of Experimental Medicine

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An important problem in chemotherapy is the development of resistance to sulfonamides both in vitro and in vivo. Spink (1), Land),. (2), and Mirick (3) have shown that when Stapkylococcus aureus and pneumococci become resistant an antisulfonamide substance is elaborated into the media by these organisms. Mirick (3) concluded that the substance produced by pneumococci was p-aminobenzoie acid (PABA) and Spink (1) and Landy (2) coneluded that the substance produced by the staphylococci was also PABA, although Spink pointed out that bioassay by the method of Lewis (4) did not yield unequivocal results. Our interest in the identity of this substance was enhanced by our previous findings (5) that certain pathogenic bacteria produce a significant quantity of another diazotizable amine during sulfonamide bacteriostasis which proved not to be PABA (6) but to which a role in the bacterial synthesis of purines has been assigned (7,8).To obtain conclusive evidence concerning the nature of the antisulfonamide substance produced by sulfa-fast staphylococci, we have attempted the isolation and characterization of this amine from culture media and found it to be PABA. E X P E R~N T A LProduction of the Amine.--A 20 liter flask was equipped with a sintered glass-tipped air inlet tube, an air outlet tube, and a sample withdrawal outlet. To this flask was added 16 liters of a solution containing NI-Ia citrate (80.0 gin.), Na~COt (40.0 gin.), NaCI (32.0 gm.), MgSO~ (3.2 gin.), KHsPO4 (48.0 gin.), and 1 liter of beef infusion broth. The flask and contents were then autoclaved for 1 hour at 20 lbs. pressure. After removal from the autoclave and while cooling, the air inlet and outlet tubes were stoppered to create a partial vacuum in the flask. When the medium had cooled to room temperature a sterile solution of glucose (64.0 gin.) in 200 ml. of water was added by immersing the sampling tube in the solution and opening its

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“…Although the method of paper chromatography has been employed successfully for the separation of amino-acids, sugars, purines, pyrimidines, phenolic compounds and othernaturallyoccurringsubstances, relatively few attempts have so far been made to apply it to the separation and identification of amines. The behaviour of various primary aromatic amines on paper chromatograms has received some attention (Ekman, 1948;Tabone, Robert & Troestler, 1948;Kelemen, Tanos & Halmagyi, 1950), but of the non-aromatic amines only the following groups of biologically important amines have been successfully separated: creatine, creatinine and their demethylated analogues (Maw, 1948;Ames & Risley, 1948); histamine and acetylhistamine (Urbach, 1949); adrenaline, noradrenaline and methyladrenaline (James, 1948); histamine, glucosamine, chondrosamine, choline, and ethanolamine (Dent, 1948;Partridge, 1948;Chargaff, Levine & Green, 1948;Aminoff, Morgan & Watkins, 1950).…”

mentioning

confidence: 99%