Separation of human X- and Y-bearing sperm using free-flow electrophoresis.

Kaneko, Satoru; Iizuka, R; Oshiro, Satoshi; Nakajima, Hiroshi; Oshio, Shigeru; Mohri, Hideo

doi:10.2183/pjab.59.276

Cited by 28 publications

(17 citation statements)

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“…Theoretically, however, the amount of H-Y antigen in X-and Y-sperm is considered to be similar. There is data [19,20] showing that human sperm can be successfully separated into two distinct populations by free-flow electrophoresis, which is based on differences in surface charge. However, there may be combined effects of surface charge and swimming characteristics when applied to separation of motile sperm.…”

Section: X-and Y-sperm Separationmentioning

confidence: 99%

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Hamano

2007

Journal of Reproduction and Development

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Abstract. Flow cytometrically-sorted sperm has been involved in the production of sex preselected offspring. More than 30,000 bovine offspring have been produced using AI and other means using spermatozoa separated by flow cytometer. Flow cytometric sperm sorting based on differences in their DNA content is the best method for separation of X-and Y-chromosome bearing spermatozoa. At first, flow cytometers were modified for DNA confirmation and sorting of sperm with high resolution. The beveled insertion needle can regulate orientation of flat-shaped bull sperm heads. The forward fluorescence detector is essential for measuring the DNA content of sperm. Recently, high-speed sperm sorting with orienting nozzles has resulted in production of 90% pure X-and Ysperm at rate of 15-20 million sperm per hour. Application of this new technique will enable conduct of more conventional technologies for both artificial insemination and cryopreservation in the bovine and in other farm animals using X-or Y-sperm.

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Section: X-and Y-sperm Separationmentioning

confidence: 99%

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Hamano

2007

Journal of Reproduction and Development

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“…These attempts have been based on numerous characteristics of the sperm, e.g., size, head shape, mass, surface charge, surface macromolecules, DNA content, and motility. Some success has been reported using free-flow electrophoresis, which separates sperm on surface charge differences (Kaneko et al, 1983) and albumin gradients, based on differences in sperm motility (Ericsonn et al, 1973). Even though these methods did produce populations enriched in Xor Y-chromosome-bearing sperm they were not successful in the fact that either motility was lost or the number of sperm recovered was negligable.…”

Section: Introductionmentioning

confidence: 99%

Separation of bovine X and Y sperm based on surface differences

Cartwright

Harrington

Cowin

et al. 1993

Molecular Reproduction Devel

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Aqueous two-phase partition involving thin-layer counter current distribution (TLCCD) has been used to assess surface heterogeneity of ejaculated bovine sperm. When partitioned in charge-insensitive aqueous two-phase systems, which detect non-charge associated surface properties, the sperm fractionates into two distinct populations. Using a Y-chromosome-specific DNA marker, it has been shown that one of these populations is enriched in Y chromosome bearing sperm. However, this population is not pure--it consists of 80% Y sperm, with the other 20% being X sperm. All the sperm in the original population that had begun to undergo the acrosome reaction were separated into this same peak; the sex chromosome composition of these sperm is unknown. Since the aqueous partition of sperm is based on surface properties these results suggest that two populations of Y sperm exist that have different surface characteristics.

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“…Th e revealed diff erences in the dynamics of thinking indices in men and women in correlation with geocosmic parameters can be explained considering innate genetic diff erences of male and female bodies. Specifi cally, it has been shown that sperm cells have diff erent electrochemical, physical and morphological characteristics depending on whether they are carrying an X-or an Y-chromosome set [5]; also, X-chromosomes are positively charged while Y-chromosomes carry negative electric charge [10].…”

Section: Resultsmentioning

confidence: 99%

Correlation of variations in geocosmic processes and thinking parameters

Volchek¹

2017

PoA

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named after the academician V.P. Kaznacheev, Russia ABSTRACTEarlier we discovered gender-specific multiyear variations in functional brain asymmetry (FBA) indices, synergistic with variations in geocosmic processes [Volchek, 2013]. FBA serves as a foundation for different types of learning, perception and processing of information. In its turn, type of learning dominating in society manifests itself in social processes, cultural events and working styles of inventors and artists. The purpose of this work was to study thinking and musical creativity indices in relation to geocosmic processes. With Torrance method, we collected data about logical, intuitive and combined thinking styles in over 3400 citizens of St. Petersburg, Russia, born in 1930-1991. Gender-specific daily, seasonal and multiyear variations in thinking indices were revealed at p≤0.001. Content analysis of 3352 Soviet songs and romances composed in 1935-1982 showed evolution of musical thinking.Of special interest is the data on multiyear variations in thinking indices in relation to geocosmic conditions. Significant and reliable variations in thinking types were discovered. For instance, the highest scores for logical thinking type (114.2) were found in men born in 1956, while the lowest scores (82.6) were found in men born in 1959, p≤0.001. According to the results of correlation analysis for the periods of 1940-1991 and 1946-1991, there Papers on Anthropology XXVI/2, 2017, pp. 182-192 Correlation of variations in geocosmic processes and thinking parameters | 183 evolution of music. Such phenomena can be reflected in social processes up to emerge of periods of so-called Thaw or Cold war.

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Separation of human X- and Y-bearing sperm using free-flow electrophoresis.

Cited by 28 publications

References 0 publications

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Separation of bovine X and Y sperm based on surface differences

Correlation of variations in geocosmic processes and thinking parameters

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