Separation of free amino acids and catecholamines in human plasma and rabbit vitreous samples using a new fluorogenic reagent 3‐(4‐bromobenzoyl)‐2‐quinolinecarboxaldehyde with CE‐LIF detection

Abstract

A sensitive and efficient analysis of amino acids and catecholamines is currently presented with 3-(4-bromobenzoyl)-2-quinolinecarboxaldehyde as a fluorogenic derivatization reagent using CE separation with LIF detection. For good derivatization conditions, the reagent concentrations, pH value, temperature, and reaction time were explored, which were followed by the derivatization reaction in stable yield. The optimal running buffer was composed of mixtures involving 120 mM, pH 9.1 boric acid, 38.5 mM SDS, and 19% ACN v/v. The LOD (S/N=3) was found as low as 0.65 nM. The proposed method was validated by the two-order-magnitude linearity and correlation coefficient ranging from 0.9957 to 0.9998. The accuracy and specificity of this assay were also assured from the spiking of real samples with a standard known concentration. In order to demonstrate its wide range of applications, the method has been applied to the analysis of both human plasma and rabbit vitreous samples.

On‐line sample preconcentration technique based on a dynamic pH junction in CE‐amperometric detection for the analysis of biogenic amines in urine

“…The six BAs chosen for this study are presented in Table . Although several reports have discussed separating BAs in acidic or neutral solution, they usually require organic additives such as SDS or ACN . According to Table , BAs are negatively charged at a pH higher than 10.20 .…”

Micellar electrokinetic chromatography: A practical overview of current methodological and instrumental advances

“…Most of the LIF–MEKC methods reported in the period covered by this review use visible lasers as the excitation source due to the wide availability of label agents that are excitable by this kind of laser. Concretely, the commercial argon‐ion laser excitation source has been consolidated and therefore used in many LIF–MEKC applications 53, 54, 143–150; few alternatives have been proposed 19, 116, with diode laser being the most interesting alternative one 36, 151–153. A large number of applications have been reported over the period covered in this review; as in the previous ones reviews 1–3, many of them involving the determination of analytes possessing no native fluorescence.…”

“…A large number of applications have been reported over the period covered in this review; as in the previous ones reviews 1–3, many of them involving the determination of analytes possessing no native fluorescence. To date, fluorescein analogues 116, 145, 146, 153, 154 and derivatives with carboxaldehyde moiety 19, 147–149 are the most widely used labelling reagents.…”

“…As stated above, many derivatization reactions have been developed to label a variety of analytes with appropriate fluorescent tags for LIF. Among them, amino acids have been a fruitful object of study 19, 116, 145, 147, 151, whereas sporadic studies have appeared on the determination of other compounds such as aliphatic 148 and biogenic 149 amines, catecholamines 53, 147, pheomelanin 146 and mercury ion 54. Many of the methods reported have used fluorescein analogues, such as FITC 116, 145, 153, 154 and 5‐carboxyfluorescein succinimidyl ester 146.…”

Simultaneous determination of monoamines in rat brain with Pt/MWCNTs@Pdop hybrid nanocomposite using capillary electrophoresis— amperometric detection

“…In recent years, CE has been developed as a powerful technique for the analysis of biological samples, due to its highly effective separation efficiency, short analysis time, low cost, and possible nanoliter sample consumption . Different detectors can be coupled with CE for the separation and detection of biogenic amines . Among them, electrochemical detection (ED) is particularly attractive as it provides high sensitivity and selectivity and without requiring laborious protocols.…”