Separation of five naphtho‐γ‐pyrones from Pleurotus ostreatus by high‐speed countercurrent chromatography

Abstract

A method to optimize a high‐speed countercurrent chromatography solvent system from many possible solvent systems was guided by average polarity and K values of crude samples, which was successfully applied in separation of the naphtho‐γ‐pyrones. This method expanded the scope of spectrophotometer application in the solvent systems selection, and showed the merits of simplicity, rapidity, and low reagent consumption. As a consequence of utilizing the method, an efficient separation procedure was established, and two groups of naphtho‐γ‐pyrone isomers (fonsecinone B and asperpyrone F; asperpyrone C, fonsecinone A, and aurasperone A) were obtained from Pleurotus ostreatus by high‐speed countercurrent chromatography with a solvent system composed of petroleum ether/ethyl acetate/methanol/water (5:5:5:5, v/v). The results indicate that high‐speed countercurrent chromatography could be a powerful technology for separation isomers and analogues, and the method guided by average polarity and K values of crude samples is feasible to optimize solvent system.

Systematic Separation and Purification of Alkaloids from Euchresta tubulosa Dunn. by Various Chromatographic Methods

“…Because too small K value will lead to HSCCC peak faster, poor separation and too large K value will lead to a wider range of peak, poor purity. According to the research and development of literature [16,17], it was found that dichloromethane system was mostly used in the separation of alkaloids by HSCCC. However, considering the difficulty of sample recovery and the convenience of experiment, it was decided not to use acidic and ionic solutions to change the solubility of water to target alkaloids, and only to add other organic reagents to change the solubility of organic to target alkaloids.…”

Rapid enrichment and separation of two novel minor phenols from Malus hupehensis utilizing liquid–liquid extraction with three‐phase solvent system and high‐speed counter‐current chromatography based on the polarity parameter

“…TPSS is usually obtained through repeated HPLC analysis, and the operation is time consuming and laborious. Moreover, it prevents the two-phase solvent system selection for high-speed counter-current chromatography (HSCCC) separation [18,19].…”

“…According to the theory of "like dissolves like," these predictive methods were connected with the polarity. The satisfactory separation of six alkaloids from Rhizoma coptidis, five naphthoγ-pyrones from Pleurotus ostreatus, and six benzaldehyde derivatives from Hypsizygus marmoreus have been oper-ated by utilizing HSCCC with solvent system selected under the guidance of the polarity [19,25,26]. Hence, the polarity was a critical parameter in characterizing a solvent system and a solute.…”

A novel foam based separation strategy for extracting minute target impurities

“…Foam separation has been used as a technique to separate mixtures based on their selective adsorption to a bubble surface. Separations of microparticles and solutes according to their foam affinity have been carried out in the past using various designs of tubular columns . In the tubing space, however, wet foams form, which carry an excess of mother liquid (the starting crude sample containing the impurities in solvent) that contaminate the foam fractions.…”

“…Others have developed methods using different columns including foam based systems to improve the separation efficiency from minor impurities but suffer from the limitations of generating complex columns . More recently, a melamine based column has been developed for efficient separation but does not use a foam based system .…”