Separation of five compounds from leaves of Andrographis paniculata (Burm. f.) Nees by off‐line two‐dimensional high‐speed counter‐current chromatography combined with gradient and recycling elution

Abstract

An off-line two-dimensional high-speed counter-current chromatography method combined with gradient and recycling elution mode was established to isolate terpenoids and flavones from the leaves of Andrographis paniculata (Burm. f.) Nees. By using the solvent systems composed of n-hexane/ethyl acetate/methanol/water with different volume ratios, five compounds including roseooside, 5,4'-dihydroxyflavonoid-7-O-β-d-pyranglucuronatebutylester, 7,8-dimethoxy-2'-hydroxy-5-O-β-d-glucopyranosyloxyflavon, 14-deoxyandrographiside, and andrographolide were successfully isolated. Purities of these isolated compounds were all over 95% as determined by high-performance liquid chromatography. Their structures were identified by UV, mass spectrometry, and (1) H NMR spectroscopy. It has been demonstrated that the combination of off-line two-dimensional high-speed counter-current chromatography with different elution modes is an efficient technique to isolate compounds from complex natural product extracts.

Target-guided separation of antioxidants from Semen cassia via off-line two-dimensional high-speed counter-current chromatography combined with complexation and extrusion elution mode

“…Column chromatography (CC) and thin layer chromatography (TLC) have been widely applied to separating NPs for centuries, but those controversial techniques have many drawbacks such as irreversible adsorption, low preparative throughput and low recovery. Recent decades, as a novel liquid-liquid partition chromatography, highspeed counter-current chromatography (HSCCC) has been widely employed for separation of bioactive compounds in NPs [16][17][18][19][20][21]. Due to using support-free liquid stationary phase, drawbacks mentioned above can be effectively eliminated [22].…”

Elution–extrusion counter‐current chromatography for the separation of two pairs of isomeric monoterpenes from Paeoniae Alba Radix

“…In recent years, highspeed counter-current chromatography (HSCCC), which can eliminate irreversible absorptive loss of samples and offers high resolution comparable to column chromatography, has been widely used in fast separation and purification of chemical components from Chinese medicinal herbs [23][24][25][26]. Further, elution-extrusion counter-current chromatography (EECCC), which combines normal elution and extrusion in a single run, was developed by Berthod [27].…”

Off‐line comprehensive two‐dimensional reversed‐phase countercurrent chromatography with high‐performance liquid chromatography: Orthogonality in separation of Polygonum cuspidatum Sieb. et Zucc

“…Combination of CCC and HPLC, also named as 2D chromatography, provides an even more robust and versatile methods for separation of complex matrix, which has been increasingly explored in separation of different natural Article Related Abbreviations: CCC, countercurrent chromatography; 1 D, first dimension; 2 D, second dimension. The heart-cut mode of 2D separation has been focused on the optimization of biphasic solvent system, improving of equipment, and the combination with the detector of ESI-MS/NMR spectroscopy [10][11][12][13][14][15][16][17][18]. On-line comprehensive 2D chromatography has been conventionally applied in gas chromatography (GC × GC) and liquid chromatography (LC×LC) and they have been well studied because of their powerful separation efficiency for complex samples [7,8].…”