Target-guided separation of antioxidants from Semen cassia via off-line two-dimensional high-speed counter-current chromatography combined with complexation and extrusion elution mode

Zeng, Hualiang; Liu, Qi; Wang, Meiling; Jiang, Shujing; Zhang, Li; He, Xi; Wang, Jun; Chen, Xiaoqing

doi:10.1016/j.jchromb.2015.07.046

Cited by 15 publications

(13 citation statements)

References 45 publications

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“…As shown in Figure 3B, seven compounds showed peak area reduction (PAR) between 13.6%–37.4%. In addition, Zeng et al reported that the rear eluting peak of the 34-min retention time is of DPPH [15]. Among these seven compounds, AM and QU (PAR 23%–37%) would be more potent antioxidants than RT, LGC, LGN, AGC and AGN, which showed PAR lower than 20.0%.…”

Section: Resultsmentioning

confidence: 99%

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Phytochemical Analysis of Agrimonia pilosa Ledeb, Its Antioxidant Activity and Aldose Reductase Inhibitory Potential

Kim

Hwang

Suh

et al. 2017

IJMS

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The aim of this study was to determine aldose reductase (AR) inhibitory activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity of compounds from Agrimonia pilosa Ledeb (AP). We isolated agrimoniin (AM), four flavonoid glucosides and two flavonoid glucuronides from the n-butanol fraction of AP 50% methanol extract. In addition to isolated compounds, the AR-inhibitory activity and the DPPH free radical scavenging activity of catechin, 5-flavonoids, and 4-flavonoid glucosides (known components of AP) against rat lens AR (RLAR) and DPPH assay were measured. AM showed IC50 values of 1.6 and 13.0 μM against RLAR and DPPH scavenging activity, respectively. Additionally, AM, luteolin-7-O-glucuronide (LGN), quercitrin (QU), luteolin (LT) and afzelin (AZ) showed high inhibitory activity against AR and were first observed to decrease sorbitol accumulation in the rat lens under high-sorbitol conditions ex vivo with inhibitory values of 47.6%, 91.8%, 76.9%, 91.8% and 93.2%, respectively. Inhibition of recombinant human AR by AM, LGN and AZ exhibited a noncompetitive inhibition pattern. Based on our results, AP and its constituents may play partial roles in RLAR and oxidative radical inhibition. Our results suggest that AM, LGN, QU, LT and AZ may potentially be used as natural drugs for treating diabetic complications.

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Section: Resultsmentioning

confidence: 99%

“…These methods required an online instrumental system and technical skills that were complex and available. Recently, the more convenient offline DPPH-HPLC method was successfully developed by spiking the complex plant extracts [15,16]. …”

Section: Introductionmentioning

confidence: 99%

Phytochemical Analysis of Agrimonia pilosa Ledeb, Its Antioxidant Activity and Aldose Reductase Inhibitory Potential

Kim

Hwang

Suh

et al. 2017

IJMS

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“…The peak area of the upper phase was recorded as A U (area of the upper phase) and that of the lower phase was recorded as A L (area of the lower phase). The K value was calculated according to the equation K = A U / A L [ 32 , 33 ].…”

Section: Methodsmentioning

confidence: 99%

Preparative Separation and Purification of Four Glycosides from Gentianae radix by High-Speed Counter-Current Chromatography and Comparison of Their Anti-NO Production Effects

et al. 2017

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Secoiridoid and iridoid glycosides are the main active components of Gentianae radix. In this work, one iridoid and three secoiridoid glycosides from Gentianae radix have been purified by high-speed counter-current chromatography in two runs using different solvent systems. Ethyl acetate–n-butanol–water (2:1:3, v/v/v) was the optimum solvent system to purify ca. 4.36 mg of loganic acid, 3.05 mg of swertiamarin, and 35.66 mg of gentiopicroside with 98.1%, 97.2% and 98.6% purities, respectively, while 31.15 mg of trifloroside with 98.9% purity was separated using hexane–ethyl acetate–methanol–water (1:3:1:3, v/v/v/v). The structures of the glycosides were identified by mass spectrometry and NMR. After separation, the anti-nitric oxide production effects of the compounds on lipopolysaccharide-induced BV-2 murine microglial cells were also evaluated. All of the compounds inhibited the production of nitric oxide in lipopolysaccharide-induced BV-2 cells with high cell viabilities in a concentration-dependent manner, which demonstrated that were able to be used as a nitric oxide inhibitor.

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“…Combination of CCC and HPLC, also named as 2D chromatography, provides an even more robust and versatile methods for separation of complex matrix, which has been increasingly explored in separation of different natural Article Related Abbreviations: CCC, countercurrent chromatography; 1 D, first dimension; 2 D, second dimension. The heart-cut mode of 2D separation has been focused on the optimization of biphasic solvent system, improving of equipment, and the combination with the detector of ESI-MS/NMR spectroscopy [10][11][12][13][14][15][16][17][18]. On-line comprehensive 2D chromatography has been conventionally applied in gas chromatography (GC × GC) and liquid chromatography (LC×LC) and they have been well studied because of their powerful separation efficiency for complex samples [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Up to 2016, 2D CCC separation of complex samples had been conducted only by heart-cut mode, that is to say, a limited number of fractions were collected from 1 D and transferred physically into 2 D separation. The heart-cut mode of 2D separation has been focused on the optimization of biphasic solvent system, improving of equipment, and the combination with the detector of ESI-MS/NMR spectroscopy [10][11][12][13][14][15][16][17][18]. However, the maximum potentiality in 2D chromatography CCC-HPLC with heart-cut mode was limited owing to the low peak capacity [19].…”

Section: Introductionmentioning

confidence: 99%

Off‐line comprehensive two‐dimensional reversed‐phase countercurrent chromatography with high‐performance liquid chromatography: Orthogonality in separation of Polygonum cuspidatum Sieb. et Zucc

Wang

Zhao

Wang

et al. 2019

J of Separation Science

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Off-line comprehensive two-dimensional reversed-phase countercurrent chromatography with high-performance liquid chromatography was investigated in separation of crude ethanol extract from traditional Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc. Two-dimensional contour plots for countercurrent chromatography with high-performance liquid chromatography was obtained after comprehensive separation was completed. Total peak capacity was evaluated and approximately 810 peaks were obtained through a comprehensive two-dimensional separation. A highly orthogonality of 52.23% and a large separation space occupancy of 88.86% were achieved.Meanwhile, it was found that several components could be well separated by countercurrent chromatography while they could not be separated by high-performance liquid chromatography, and vice versa, which further indicated the orthogonality of the two separation methods. The off-line comprehensive two-dimensional countercurrent chromatography with high-performance liquid chromatography provided a promising and powerful method for separation of complex natural products. K E Y W O R D Scountercurrent chromatography, comprehensive two-dimensional chromatography, high performance liquid chromatography, orthogonality, traditional Chinese medicine

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Target-guided separation of antioxidants from Semen cassia via off-line two-dimensional high-speed counter-current chromatography combined with complexation and extrusion elution mode

Cited by 15 publications

References 45 publications

Phytochemical Analysis of Agrimonia pilosa Ledeb, Its Antioxidant Activity and Aldose Reductase Inhibitory Potential

Phytochemical Analysis of Agrimonia pilosa Ledeb, Its Antioxidant Activity and Aldose Reductase Inhibitory Potential

Preparative Separation and Purification of Four Glycosides from Gentianae radix by High-Speed Counter-Current Chromatography and Comparison of Their Anti-NO Production Effects

Off‐line comprehensive two‐dimensional reversed‐phase countercurrent chromatography with high‐performance liquid chromatography: Orthogonality in separation of Polygonum cuspidatum Sieb. et Zucc

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