Separation of enantiomers on a lysozyme-bonded silica column

Chiral separation by chromatographic and electromigration techniques. A Review

“…Two cellobiohydrolases, CBH I and CBH II [222] and more recently CBH 58 [223], found application as chiral selectors for the preparation of silica-based CSPs which showed remarkable enantioselectivity for a broad spectrum of drugs, among them b-blockers [224]. Lysozyme [225]-and pepsin [226]-based CSPs showed enantioselectivity for basic and neutral enantiomers but not for acidic enantiomers. Recently, the preparation of amyloglucosidasebased CSPs and its application to aminoalcohols was reported [227].…”

Separation of drug enantiomers by liquid chromatography and capillary electrophoresis, using immobilized proteins as chiral selectors

“…It is worth noting that proteins, such as riboflavin binding protein (RfBP) [28], ovoglycoprotein (the active fraction of crude ovomucoid preparations) [29] and amyloglucosidase [30] are promising candidates for the chiral separation of basic drugs. Other chiral stationary phases using immobilized trypsin [31], ␣-chymotrypsin [32], conalbumin (ovotransferrin) [33] and lysozyme [34] have been described, even if their applicability is more limited.…”

Study of physically adsorbed stationary phases for open tubular capillary electrochromatography

“…Proteins, peptides and amino acids have been used as chiral selectors (including chiral stationary phase and chiral additive for mobile phase) in HPLC [40,41], CE [42± 45], OTCEC, and OTLC [27]. One important research interest is to develop a chiral stationary phase from these substances through the adsorption effect.…”