Separation of Enantiomers of Drugs by Capillary Electrophoresis, Part 6. Hydroxypropyl-β-cyclodextrin as Chiral Solvating Agent

Lin, Bingcheng; Epperlein, Ulrich; Schwierskott, Marc; Schlunk, Rainer; Koppenhoefer, Bernhard

doi:10.1002/(sici)1521-4168(19980401)21:4<215::aid-jhrc215>3.3.co;2-u

Cited by 4 publications

(4 citation statements)

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“…This method is the most efficient separation technique available for the analysis of both large and small molecules. There are six types of capillary electro separation available but the most commonly used technique seems to be CZE, which is widely used for analyses of various substances, including active pharmaceutical ingredients (either in formulations or biological fluids), drug counter ions . Taking this into account we decided to use this method to detection and separation of compounds with biological activity.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.0^2,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs

Grodner

Łukaszkiewicz

Kuran

et al. 2014

J of Separation Science

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The purpose of this study, the direct separation of aminoalkanol derivatives I and II of 1,7-dimethyl-8,9-diphenyl-4-azatricyclo[5.2.1.0(2,6) ]dec-8-ene-3,5,10-trione, which was found in earlier studies as potential anticancer drugs, were performed. Capillary electrophoresis offers the possibility of fast, cheap, and reproducible separations for compounds I and II. In this paper, the simultaneous separation of I and II by capillary zone electrophoresis has been achieved within 8 min by use of 50 mM phosphate buffer of pH 2.5. Analysis of the two compounds in the serum plasma standards was conducted. Limits of detection of I and II by UV absorbance at 200 nm were achieved in the range of 156.3-156.6 ng/mL. The method was validated for linearity, accuracy, precision, limits of detection, and quantification. The calibration equation revealed a good linear relationship (r(2) = 0.998-0.999). Sufficient recovery was observed in the range of 96.3-99.5%. The method showed good reproducibility with intra- and interday precision of 0.97 and 1.76%, respectively. The quantification limits for the compounds were in the range of 477.0-479.8 ng/mL. The proposed method was applied to the analysis of real serum samples.

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Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.0^2,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs

Grodner

Łukaszkiewicz

Kuran

et al. 2014

J of Separation Science

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“…The data set of CE MT was taken from the values reported by Bingcheng et al 30. The molecules in the data set including their MT values are given in Table 1.…”

Section: Methodsmentioning

confidence: 99%

A novel QSPR study of normalized migration time for drugs in capillary electrophoresis by new descriptors: Quantum chemical investigation

2008

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Some drugs' migration time (MT) has been studied employing quantitative structure-property relationship using new descriptors that are able to predict MT value with high accuracy. MT property modeling of the drugs was established as a function of the new theoretically derived descriptors applying multiple linear regressions and partial least-squares regression. The genetic algorithm was used to select those variables that resulted in the best-fitted models. To select a set of descriptors that are most relevant to MT, illustrating the affecting degree for the affinity of different descriptors, the linear models with 1-14 variables were constructed and were then investigated based on F-value, squared regression coefficients of cross-validated (Q2), adjusted R2 (R2adj) and standard error of estimate (S) statistical parameters. Finally, the best model with ten variables was selected. Statistical parameters of the test set, such as standard deviation error in test, were 0.559 and 0.616, while relative error of test was equal to 7.648 and 8.497% for multiple linear regressions and partial least-squares models, respectively, confirming the good predictive ability of the model. Since the capillary lengths were not the same for the drugs in the data set, MT values were normalized based on a specific capillary before modeling, which is also one of the advantages of this method, enabling us to use the model for different capillary lengths.

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“…A comprehensive study of the effect of nature of CD on enantioresolution had been undertaken in a series of works by Koppenhoefer et al. using ofloxacin as a prober. Table summarizes their results in terms of migration enantioselectivity coefficient α m .…”

Section: Chiral Separation By Cementioning

confidence: 99%

Chiral separation of quinolones by liquid chromatography and capillary electrophoresis

2017

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The quinolones are derivatives of oxoquinolines and mostly known for their antibacterial and antiviral activities. Many quinolones are chiral compounds having asymmetric centers and important due to their enantioselective biological activities. In order to study the biological activities of quinolone enantiomers, to control the manufacturing of homochiral drugs and to prepare necessary quantities of pure enantiomers for preclinical or clinical trials, respective chiral separation methods are urgently needed. In this context, the present review discusses chromatographic and electrophoretic methods for the enantioseparation of chiral quinolones and provides some useful information on their physical and pharmaceutical properties. The drawbacks of currently used techniques are revealed and ways to overcome them are outlined. Moreover, recommendations for an optimal choice of a separation protocol are given.

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Separation of Enantiomers of Drugs by Capillary Electrophoresis, Part 6. Hydroxypropyl-β-cyclodextrin as Chiral Solvating Agent

Cited by 4 publications

References 0 publications

Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.0^2,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs

Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.0^2,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs

A novel QSPR study of normalized migration time for drugs in capillary electrophoresis by new descriptors: Quantum chemical investigation

Chiral separation of quinolones by liquid chromatography and capillary electrophoresis

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Separation of Enantiomers of Drugs by Capillary Electrophoresis, Part 6. Hydroxypropyl-β-cyclodextrin as Chiral Solvating Agent

Cited by 4 publications

References 0 publications

Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.02,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs

Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.02,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs

A novel QSPR study of normalized migration time for drugs in capillary electrophoresis by new descriptors: Quantum chemical investigation

Chiral separation of quinolones by liquid chromatography and capillary electrophoresis

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Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.0^2,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs

Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.0^2,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs