Chiral separation of quinolones by liquid chromatography and capillary electrophoresis

Abstract

The quinolones are derivatives of oxoquinolines and mostly known for their antibacterial and antiviral activities. Many quinolones are chiral compounds having asymmetric centers and important due to their enantioselective biological activities. In order to study the biological activities of quinolone enantiomers, to control the manufacturing of homochiral drugs and to prepare necessary quantities of pure enantiomers for preclinical or clinical trials, respective chiral separation methods are urgently needed. In this context, the present review discusses chromatographic and electrophoretic methods for the enantioseparation of chiral quinolones and provides some useful information on their physical and pharmaceutical properties. The drawbacks of currently used techniques are revealed and ways to overcome them are outlined. Moreover, recommendations for an optimal choice of a separation protocol are given.

Structure‐retention relationship for enantioseparation of selected fluoroquinolones

“…Hazardous side effects are usually encountered for inactive enantiomers, owing to dissimilar mechanism of metabolism, excretion, or clearance . To that end, several chromatographic methods have reviewed for enantiomeric separation of fluoroquinolones . Most HPLC methods used indirect strategies either by derivatization of GFLX and OFLX or by addition of cyclodexrin in the mobile phase for enantiomeric separation of NFLX .…”

“…6,7 To that end, several chromatographic methods have reviewed for enantiomeric separation of fluoroquinolones. [8][9][10][11] Most HPLC methods used indirect strategies either by derivatization of GFLX 12 and OFLX [13][14][15][16][17][18][19] or by addition of cyclodexrin in the mobile phase for enantiomeric separation of NFLX. 20,21 Some other researches applied direct enantiomeric separations by using chiral stationary phases (CSPs) like amylosebased CSP 22,23 for GFLX.…”

Progress in Polymeric Nano-Medicines for Theranostic Cancer Treatment

“…These were The number of publications in polymer therapeutics is increasing continuously. During our research of more than 30 years and available literature on cancer treatment [2][3][4][5][6][7][8][9][10][11][12][13][14][15], it was observed that chemotherapy is considered the best approach to curb different types of cancers [16][17][18][19][20][21][22][23][24][25][26][27][28]. But this has severe toxicity to normal cells.…”

Chiral separation and modeling of quinolones on teicoplanin macrocyclic glycopeptide antibiotics CSP

“…Once the importance of chirality in bioactivities of quinolone‐based drugs was recognized, the efforts are made to develop analytical techniques to quantify their enantiomeric composition. A thorough search of literature on the enantiomeric resolution of quinolones was carried out through Scifinder, Scopus, and Science direct and peer reviewed Journals . It was observed that there are only few methods available of the chiral separation of these drugs on chiral cwon ether, proteins, and polysaccharide, but there is no paper on the most effective macrocyclic glycopeptides antibiotics Chiral Stationary Phase (CSPs).…”

“…A thorough search of literature on the enantiomeric resolution of quinolones was carried out through Scifinder, Scopus, and Science direct and peer reviewed Journals. [30][31][32][33][34] It was observed that there are only few methods available of the chiral separation of these drugs on chiral cwon ether, proteins, and polysaccharide, but there is no paper on the most effective macrocyclic glycopeptides antibiotics Chiral Stationary Phase (CSPs). In view of these facts, the efforts were made to develop fast, reproducible, and effective high-performance liquid chromatography (HPLC) methods using macrocyclic glycopeptides antibiotics CSPs.…”