Separation of compounds interacting with liposome membrane in combined prescription of traditional Chinese medicines with immobilized liposome chromatography

Recent analytical approaches in quality control of traditional Chinese medicines—A review

“…Through these methods, many Chinese crude drugs or their combined prescriptions were studied, including Ligusticum chuanxiong, Coptis chinensis, Rheum palmatum, Angelica sinensis, Longdan Xiegan decoction (LXD), Danggui Buxue decoction (DBD), etc. [10][11][12][13][14][15].…”

“…Although these methods have been approved to be useful approaches for rapid screening and analysis of bioactive candidates from TCMs [10][11][12][13][14][15], some limitations should be noticed, such as only one type of target has been immobilized in the solid phase (by example HAS only), so the possible active compounds binding with the other targets may be lost; the life of immobilized target is very short, and the entrapment or immobilization procedure must be adapted to the material analyzed and to the gel matrix used [16]; the separation ability of the affinity chromatography is lower than that of conventional HPLC so that several compounds may be coeluted as one peak, and this method cannot be directly coupled with MS due to the limitation of the mobile phase, resulting in difficulty of on-line identification of the retention components.…”

Liposomes in chromatography

“…The flow-rate was set to 1.0 mL/min and analytes were detected at 210 nm. The measurements indicated that ligustilide, astragaloside IV and formonoteinin bound strongly to membrane while the binding of ferulic acid was fairly weak (Sheng et al, 2005). The interaction between tetraphenylphosphonium and triphenylphosphonium homologs with liposomes was also investigated by ILC.…”

Screening and identification of potential bioactive components in a combined prescription of Danggui Buxue decoction using cell extraction coupled with high performance liquid chromatography

“…ferulic acid) were thought to be the main bioactive compounds in DBD (Ma et al, 2002;Qi et al, 2006;Liu et al, 2002;Chen et al, 2006). Astragaloside IV (13), formononetin (10) and ligustilide (12) were considered as the main bioactive ingredients in DBD obtained by ILC (Sheng et al, 2005;Mao et al, 2002). Calycosin-7-O-β-D-glucoside (1), senkyunolide I or H (4), ononin (5), (6aR,11aR)-9,10-dimethoxypterocarpan-3-O-β-D-glucoside, (3R)-29-hydroxy-39,49-dimethoxyisoflavan-7-O-β-D-glucoside (6), calycosin (7), astragaloside IV (13), isoastragaloside II, formononetin (10), (6aR,11aR),-3-hydroxy-9,10-dimethoxypterocarpan (8), (3R)-7,29-dihydroxy-39,49- (12) were considered as the permeable components which interact with the liposome membranes .…”