Separation of cobalt binding proteins by immobilized metal affinity chromatography

Ionic liquid-based aqueous two-phase extraction of selected proteins

“…The salting-out ability can also be related to the Gibbs energy of hydration of the ions. Because these salts share a common cation but contain different anions, it is easy to find that the salting-out ability of these anions follows the order: PO 4 3− ( G hyd = −2765 kJ mol −1 ) > CO 3 2− ( G hyd = −1315 kJ mol −1 ) > OH − ( G hyd = −430 kJ mol −1 ) [28]. This order follows the Hofmeister series for the strength of the kosmotropic salts.…”

“…Traditional methods to purify proteins involve several steps such as ammonium sulfate precipitation, ionic and affinity chromatography [3], or electrophoresis [4]. However, some of these separation techniques are time and cost consuming.…”

Design of ionic liquids for lipase purification

“…Moreover, when these processes are applied to biological materials, rigorous purification steps, delicate enough to preserve the biological activity, are required. The most common techniques used are the ammonium sulphate precipitation [7], ionic and affinity chromatography [8,9], dialysis, filtration, electrophoresis [10][11][12][13] and reverse micelles approaches [14,15]. Nevertheless, some of these separation processes are costly and time consuming, and are not easily scalable [7].…”

Selective enrichment of metal-binding proteins based on magnetic core/shell microspheres functionalized with metal cations

“…12 Hence, a large number of IMAC strategies have been developed in the case of pre-fractionate proteins for metalloproteomic investigation by immobilizing different metal ions, 13 such as Zn 2+ , Ni 2+ , Cu 2+ and Co 2+ . [14][15][16] By using chelating Sepharose Fast Flow resin (Amersham Biosciences), She et al 17 analysed Cu and Zn metalloproteomes in three human hepatoma cell lines (Hep G2, Mz-Hep-1 and SKHep-1), and Smith et al 18 identified 67 copper-binding proteins in the cytoplasmic and microsomal fractions of Hep G2 cells. Wang et al 19 carried out a systematic screening for copper-binding proteins in soybean seeds by using IMAC technology.…”