Ionic liquid-based aqueous two-phase extraction of selected proteins

Extraction of vanillin using ionic-liquid-based aqueous two-phase systems

“…While He et al [20] indicated that temperature had not significant influence on the distribution behavior of steroids, Pei et al [23], on the other hand, reported that temperature greatly influences the extraction efficiency of proteins. As a result it can be established that the partition coefficients dependence on temperature will largely depend on the solute and ternary system under study.…”

“…The effect of temperature on the partition coefficients of vanillin was studied with four ILs: [C 4 [20,23,[35][36], using IL-based ATPS, the temperature was shown to be either a negligible or a significant factor in the extraction of various types of biomolecules. While He et al [20] indicated that temperature had not significant influence on the distribution behavior of steroids, Pei et al [23], on the other hand, reported that temperature greatly influences the extraction efficiency of proteins.…”

“…It was already shown that ATPS formed by K 3 PO 4 and ILs are extremely advantageous for the partition of several biomolecules yielding larger partition coefficients than those conventionally obtained with polymer-inorganic salts or polymer-polymer ATPS [6,10,13]. The extractive potential of biomolecules using IL-based ATPS was previously studied for distinct compounds, such as testosterone and epitestosterone, alkaloids, antibiotics, bovine serum albumin, penicillin G, ltryptophan and food coulorants [6,10,13,[20][21][22][23][24].…”

Ionic Liquids in Separation Techniques

“…ATPS have been used mainly in biotechnology for the separation of biomolecules, such as cells, organelles, membrane fractions and proteins (Walter & Johansson, 1994;Albertsson, 1986;Rito-Palomares, 2004;Gunduz &Tolga, 2004;Roobol-Boza et al, 2004;Agasøster, 1998).This method appears to be an attractive alternative to conventional extraction methods such as liquid-liquid extraction and solid-phase extraction, to simultaneously carry out isolation, purification and enrichment of biomolecules (RitoPalomares, 2004;Gunduz & Tolga, 2004;Roobol-Boza et al, 2004). Apart from proteins like bovine serum albumin, lysozyme, trypsin, myoglobin (Pei et al, 2009, Du et al, 2007Dreyer & Kragl, 2008;Dreyer and Kragl, 2009) ATPS have been used for the recovery of small organic and inorganic molecules such as metal ions, radiochemicals, dyes, drug molecules (Huddleston et al, 1999;Willauer et al, 2002;Zhu et al, 2001;Bridges & Rogers, 2008). Short chain alcohols (Gutowski et al, 2003), phenol (Chen et al, 2009), testosterone and epitestosterone (He et al, 2005), penicillin G , opium alkaloids (Li et al, 2005a), L-tryptophan Ventura et al, 2009;Louros et al, 2010), antibiotics (Dominguez-Perez et al, 2010), caffeine and nicotine have been analysed as partitioning solutes so far.…”

Determination of three physical properties of quinoline ionic liquids with hexafluorophosphate

“…Despite the great possibilities in application of the IL-PEG system, there are limited reports about their properties or fundamental data [4,5]. Recently, ILs shows great prospect as extraction solvents in liquidliquid extraction, solid-liquid extraction and liquid-phase extraction because of their good solubility property [6,7], and they play an important role in extracting compounds such as protein [8], heavy-metal ions [9], traditional Chinese medicine from complicated samples [10], and they can improve selectivity remarkably as well as efficiency. Furthermore, it was reported that waterimmiscible BF 4 -based ILs can be used in the extraction of metal ions from wasted water [11].…”