Separation of chromosomal DNA molecules from Phoma tracheiphila by orthogonal-field-alternation gel electrophoresis

Rollo, Franco; Ferracuti, Tina; Pacilli, A.

doi:10.1007/bf00340730

Cited by 9 publications

(1 citation statement)

References 7 publications

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“…The assignment of linkage groups to the resolved chromosomal DNA bands may be undertaken using linkage group-specific probes. The physical analysis of fungal genomes by OFAGE has been successful with other plant pathogenic fungi as an aid to karyotyping or genomic mapping (Kinscherf & Leong, 1988;Rollo et al, 1989;Tzeng et af., 1989). Recently, chromosome length polymorphisms were observed among different races of Ustilago hordei Samac & Leong, 1989).…”

Section: Discussionmentioning

confidence: 99%

Unique DNA plasmid pRS64 associated with chromosomal DNAs of the plant pathogenic fungus Rhizoctonia solani

Wako

Ishikawa

Hashiba

1991

Journal of General Microbiology

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Unique DNA sequences homologous to the linear DNA plasmid pRS64 were investigated in chromosomal DNAs of isolates belonging to anastomosis group 4 (AG-4) of the plant pathogenic fungus Rhizoctonia sofani. Chromosome-sized DNAs of isolates RI-64 and 1271 of AG-4 were separated into six bands by orthogonal-fieldalternation gel electrophoresis and hybridized to a cloned segment of pRS64. A small chromosome-sized DNA band of approximately 1.1 Mb carried the sequences homologous to pRS64 DNA. Sequences homologous to pRS64 were also maintained within the chromosomal DNA of isolate 1271 of AG-4 which does not possess the plasmid. The plasmid showed no homology to the mitochondrial DNA of isolate 1271. The possibility that the linear plasmid pRS64 may act as a transposable genetic element is discussed.

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Section: Discussionmentioning

confidence: 99%