Molecular Genetics of Phytopathogenic Fungi

Tudzynski, Paul; Weltring, Klaus-Michael

doi:10.1007/978-3-642-78020-2_20

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“…Prolonged transit or storage in packing houses at low temperature (below 20 C) could highly promote this type of decay because this pathogen is able to grow and produce abundant sporulation at 0 C (Michailides, 1980). Different types of molecular markers are available for use in evolutionary and population studies of phytopathogenic fungi (Michelmore and Hulbert, 1987;Tudzynski and Weltring, 1993). Among the methods applied, isozyme analysis on starch or polyacrylamide electrophoresis provides a well-established and efficient tool for revealing genetic variability in fungal populations (Micales et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Isozyme Variation among Isolates of Mucor piriformis

Vágvölgyi¹,

Papp²,

Palagyi³

et al. 1996

Mycologia

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Section: Introductionmentioning

confidence: 99%

Isozyme Variation among Isolates of Mucor piriformis

Vágvölgyi¹,

Papp²,

Palagyi³

et al. 1996

Mycologia

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Papp¹,

Vágvölgyi²,

Kerényi

et al. 1997

Antonie Van Leeuwenhoek

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Mucor piriformis can cause postharvest decay in various fruits and vegetables stored at low temperatures. Thirty isolates of this fungus, collected from infected fruit, were subjected to random amplified polymorphic DNA (RAPD) analysis. Seven different 10-bp primers were used to determine the type and extent of intraspecific genetic polymorphisms. Nineteen composite amplification types were identified, indicating a higher degree of variability than found in previous isoenzyme studies. Numerical analysis with the UPGMA technique revealed three clusters, which correlated with the mating competency of the isolates or their place of origin. These results demonstrate that RAPD analysis can identify isolates and subspecific populations of M. piriformis.

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Molecular Genetics of Phytopathogenic Fungi

Cited by 2 publications

References 95 publications

Isozyme Variation among Isolates of Mucor piriformis

Isozyme Variation among Isolates of Mucor piriformis

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