Separation of cardiac glycosides by micellar electrokinetic chromatography and microemulsion electrokinetic chromatography

Debusschere, L.; Demesmay, Claire; Rocca, J. L.; Lachâtre, Gérard; Lofti, Hayat

doi:10.1016/s0021-9673(97)00413-5

Cited by 39 publications

(23 citation statements)

References 18 publications

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“…[49] Insoluble ingredients of pharmaceutical ointment 0.81% w/w n-octane 3.97% w/w SDS 6.61% w/w butan-1-ol 10% w/w propan-2-ol 78.61% w/w 10 mM sodium tetraborate [43] Cephalosporins 0.82% w/w n-heptane 1.44% w/w glycodeoxycholic acid (GDC) 5.68% w/w Tween 6.49% w/w butan-1-ol 10 mM phosphate buffer (pH 7.0) [39] Cardiac glycosides 0.81% w/w n-heptane 1.66%w/w SDS 6.61% w/w butan-1-ol 90.92% w/w borate buffer [34] Steroids 0.81% w/w n-hexanol 3.31% w/w SDS 6.61% w/w butan-1-ol 89.28% w/w 20 mM phosphate buffer (pH 10.0) [29] 41 mM n-heptane 100 mM SDS 700 mM butanol 50 mM phosphoric acid (pH 1.9)…”

Section: Effect Of Phmentioning

confidence: 99%

Background and operating parameters in microemulsion electrokinetic chromatography

2003

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Microemulsion electrokinetic chromatography (MEEKC) is an electrodriven separation technique. Separations are generally achieved using microemulsions consisting of surfactant-coated nanometer-sized oil droplets suspended in aqueous buffer. A cosurfactant such as a short-chain alcohol is generally used to stabilize the microemulsion. This review summarizes the various microemulsion types and compositions that have been used in MEEKC. The effects of key-operating variables such as surfactant type and concentration, cosurfactant type and concentration, buffer pH and type, oil type and concentration, use of organic solvent and cyclodextrin additions, and temperature are described. Specific examples of water-in-oil microemulsions and chirally selective separations are also covered.

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Section: Effect Of Phmentioning

confidence: 99%

Background and operating parameters in microemulsion electrokinetic chromatography

2003

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“…Early applications were in the separation of pharmaceutical substances such as steroids [11,34,40,48,62], cardiac glycosides [85], analgesics [52] and cephalosporins [86]. More recent applications have seen the analysis of pharmaceutical formulations.…”

Section: Pharmaceutical Analysismentioning

confidence: 99%

Recent advances in the development and application of microemulsion EKC

et al. 2007

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Microemulsion EKC (MEEKC) is an electrodriven separation technique. Separations are typically achieved using oil-in-water microemulsions, which are composed of nanometre-sized oil droplets suspended in an aqueous buffer. The droplets are stabilised by a surfactant and a cosurfactant. The novel use of water-in-oil microemulsions has also been investigated. This review summarises the advances in the development of MEEKC separations and also the different areas of application including determination of log P values, pharmaceutical applications, chiral analysis, natural products and bioanalytical separations and the use of new methods such as multiplexed MEEKC and high speed MEEKC. Recent applications (2004-2006) are tabulated for each area with microemulsion composition details.

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“…Neutral substances like steroids were separated in standard MEEKC systems at pH 7-11 [6]. Fu et al [25] separated four analgesics, Debusschère et al [11] separated cardiac glycosides, and Mrestani et al [10] separated cephalosporins, but in none of these investigations analyses of real samples like drug formulations were performed. Altria and co-workers have published a series of papers [18,[26][27][28][29][30] with a number of applications in drug analysis.…”

Section: Pharmaceuticalsmentioning

confidence: 99%

Recent applications of microemulsion electrokinetic chromatography

Hansen

2003

Electrophoresis

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Microemulsions are used in a similar way as micellar solutions are used for separations in capillary electrophoresis. Within the last approximately six years, a number of papers have appeared in the literature in which the separation characteristics of microemulsion electrokinetic chromatography (MEEKC) have been investigated. The total number of papers dealing with MEEKC is now close to 100. One of the major fields of application for MEEKC has been the characterization of the lipophilicity of chemical substances but the technique has also been applied for the analysis of substances within the fields of natural products, pharmaceuticals, vitamins, peptides, proteins, and nucleic acid bases as well as nucleosides. An overview of the applications is given together with the microemulsions used for each application.

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Separation of cardiac glycosides by micellar electrokinetic chromatography and microemulsion electrokinetic chromatography

Cited by 39 publications

References 18 publications

Background and operating parameters in microemulsion electrokinetic chromatography

Background and operating parameters in microemulsion electrokinetic chromatography

Recent advances in the development and application of microemulsion EKC

Recent applications of microemulsion electrokinetic chromatography

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