Separation of blood cells using hydrodynamic lift

Geislinger, Thomas M.; Eggart, B.; Braunmüller, Susanne; Schmid, Lothar; Franke, Thomas

doi:10.1063/1.4709614

Cited by 59 publications

(45 citation statements)

References 27 publications

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“…But there are few works where cell separation microdevices are solely designed based on the deformabilityinduced cross migration. For example, Geislinger et al 128 separated RBCs from other blood components by harnessing deformability-selective cell-wall interaction in a non-inertial flow inside a microchannel (Fig. 4(a)).…”

Section: Deformability-selective Cell Separationmentioning

confidence: 99%

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

2013

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Focusing and sorting cells and particles utilizing microfluidic phenomena have been flourishing areas of development in recent years. These processes are largely beneficial in biomedical applications and fundamental studies of cell biology as they provide cost-effective and point-of-care miniaturized diagnostic devices and rare cell enrichment techniques. Due to inherent problems of isolation methods based on the biomarkers and antigens, separation approaches exploiting physical characteristics of cells of interest, such as size, deformability, and electric and magnetic properties, have gained currency in many medical assays. Here, we present an overview of the cell/particle sorting techniques by harnessing intrinsic hydrodynamic effects in microchannels. Our emphasis is on the underlying fluid dynamical mechanisms causing cross stream migration of objects in shear and vortical flows. We also highlight the advantages and drawbacks of each method in terms of throughput, separation efficiency, and cell viability. Finally, we discuss the future research areas for extending the scope of hydrodynamic mechanisms and exploring new physical directions for microfluidic applications.

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Section: Deformability-selective Cell Separationmentioning

confidence: 99%

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

2013

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“…For a fixed shape, Uðk; r 1 ; r 2 Þ decreases with increasing viscosity ratio k. 26 This prediction has been shown to be in qualitative agreement with experiments and theoretical results for deformable lipid vesicles in microgravity 42,43 and for RBCs and platelets in a Poiseuille flow. 39 …”

Section: A Non-inertial Hydrodynamic Liftmentioning

confidence: 99%

“…The RBCs start a tank-treading motion when affected by the sheath flow. 39,45,47,48 This includes a stable inclination angle of the RBCs when passing x 1 and results in an entrance height of z RBC_x1 ¼ (8 6 2) lm. The centers of the cell distributions are already segregated with the RBCs below the melanoma cells while the distributions itself still overlap clearly.…”

Section: -4mentioning

confidence: 99%

Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift

Geislinger

Franke

2013

Biomicrofluidics

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We demonstrate the method of non-inertial lift induced cell sorting (NILICS), a continuous, passive, and label-free cell sorting approach in a simple single layer microfluidic device at low Reynolds number flow conditions. In the experiments, we exploit the non-inertial lift effect to sort circulating MV3-melanoma cells from red blood cell suspensions at different hematocrits as high as 9%. We analyze the separation process and the influence of hematocrit and volume flow rates. We achieve sorting efficiencies for MV3-cells up to E MV3 ¼ 100% at Hct ¼ 9% and demonstrate cell viability by recultivation of the sorted cells. V C 2013 AIP Publishing LLC. [http://dx

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“…Maenaka et al [32] demonstrated this size-dependent separation scheme for binary droplets in a pinched channel. Geislinger et al [33] exploited the separation of red blood cells from other blood components in a simple T-shaped channel. Using the same channel, sorting of the circulating MV3-melanama cells from suspension of red blood cells was also achieved [34].…”

Section: Introductionmentioning

confidence: 99%

Lateral migration of dual droplet trains in a double spiral microchannel

Xue

Chen

Liu

et al. 2016

Sci. China Phys. Mech. Astron.

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Microfluidic droplets have emerged as novel platforms for chemical and biological applications. Manipulation of droplets has thus attracted increasing attention. Different from solid particles, deformable droplets cannot be efficiently controlled by inertia-driven approaches. Here, we report a study on the lateral migration of dual droplet trains in a double spiral microchannel at low Reynolds numbers. The dominant driving mechanism is elucidated as wall effect originated from the droplet deformation. Three types of migration modes are observed with varying Reynolds numbers and the size-dependent mode is intensively investigated. We obtain empirical formulas by relating the migration to Reynolds numbers and droplet sizes. The effect of droplet deformability on the migration and the detailed migration behavior along the double spiral channel are discussed. Numerical simulations are also performed and yielded in qualitative agreement with the experiments. This proposed low Re approach based on lateral migration could be a promising alternative to existing inertia-driven approaches especially concerning deformable entities and susceptible bio-particles.

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Separation of blood cells using hydrodynamic lift

Cited by 59 publications

References 27 publications

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift

Lateral migration of dual droplet trains in a double spiral microchannel

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