Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift

Geislinger, Thomas M.; Franke, Thomas

doi:10.1063/1.4818907

Cited by 46 publications

(36 citation statements)

References 47 publications

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“…The current techniques proposed for particle separation rely on forcing the particles to move at different velocities but in the same direction. Other separation techniques based on sizedependent hydrodynamical long-range interactions 3,12 or ratcheting of particles moving in asymmetric arrays or channels have been suggested [13][14][15][16][17][18][19][20][21][22] .…”

Section: Introductionmentioning

confidence: 99%

Optimizing the performance of the entropic splitter for particle separation

Motz

Schmid

Hänggi

et al. 2014

The Journal of Chemical Physics

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Recently, it has been shown that entropy can be used to sort Brownian particles according to their size. In particular, a combination of a static and a time-dependent force applied on differently sized particles which are confined in an asymmetric periodic structure can be used to separate them efficiently, by forcing them to move in opposite directions. In this paper, we investigate the optimization of the performance of the "entropic splitter". Specifically, the splitting mechanism and how it depends on the geometry of the channel, and the frequency and strength of the periodic forcing is analyzed. Using numerical simulations, we demonstrate that a very efficient and fast separation with a practically 100% purity can be achieved by a proper optimization of the control variables. The results of this work could be useful for a more efficient separation of dispersed phases such as DNA fragments or colloids dependent on their size.

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Section: Introductionmentioning

confidence: 99%

Optimizing the performance of the entropic splitter for particle separation

Motz

Schmid

Hänggi

et al. 2014

The Journal of Chemical Physics

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“…(4). Other properties such as stiffness 68,69 are not considered in this work for rigid particles, which may be potential markers for the C-iDEP sorting of disease infected cells. We have also developed a numerical model to understand the shape-based particle sorting in the spiral microchannel, which predicts qualitatively the experimentally observed deflection behaviors of each type of particles in the two spirals.…”

Section: Discussionmentioning

confidence: 97%

Microfluidic electrical sorting of particles based on shape in a spiral microchannel

DuBose

Patel

et al. 2014

Biomicrofluidics

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Shape is an intrinsic marker of cell cycle, an important factor for identifying a bioparticle, and also a useful indicator of cell state for disease diagnostics. Therefore, shape can be a specific marker in label-free particle and cell separation for various chemical and biological applications. We demonstrate in this work a continuous-flow electrical sorting of spherical and peanut-shaped particles of similar volumes in an asymmetric double-spiral microchannel. It exploits curvature-induced dielectrophoresis to focus particles to a tight stream in the first spiral without any sheath flow and subsequently displace them to shape-dependent flow paths in the second spiral without any external force. We also develop a numerical model to simulate and understand this shape-based particle sorting in spiral microchannels. The predicted particle trajectories agree qualitatively with the experimental observation.

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“…devised a hydrodynamic lift system enhanced by hydrodynamic spreading to separate CTC and red blood cells. 177, 178 …”

Section: Label-free Cell Sortingmentioning

confidence: 99%

Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation

2015

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Accurate and high throughput cell sorting is a critical enabling technology in molecular and cellular biology, biotechnology, and medicine. While conventional methods can provide high efficiency sorting in short timescales, advances in microfluidics have enabled the realization of miniaturized devices offering similar capabilities that exploit a variety of physical principles. We classify these technologies as either active or passive. Active systems generally use external fields (e.g., acoustic, electric, magnetic, and optical) to impose forces to displace cells for sorting, whereas passive systems use inertial forces, filters, and adhesion mechanisms to purify cell populations. Cell sorting on microchips provides numerous advantages over conventional methods by reducing the size of necessary equipment, eliminating potentially biohazardous aerosols, and simplifying the complex protocols commonly associated with cell sorting. Additionally, microchip devices are well suited for parallelization, enabling complete lab-on-a-chip devices for cellular isolation, analysis, and experimental processing. In this review, we examine the breadth of microfluidic cell sorting technologies, while focusing on those that offer the greatest potential for translation into clinical and industrial practice and that offer multiple, useful functions. We organize these sorting technologies by the type of cell preparation required (i.e., fluorescent label-based sorting, bead-based sorting, and label-free sorting) as well as by the physical principles underlying each sorting mechanism.

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Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift

Cited by 46 publications

References 47 publications

Optimizing the performance of the entropic splitter for particle separation

Optimizing the performance of the entropic splitter for particle separation

Microfluidic electrical sorting of particles based on shape in a spiral microchannel

Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation

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