Separation of bile acids by column-partition chromatography

Mosbach, Erwin H.; Zomzely, Claire; Kendall, Forrest E.

doi:10.1016/0003-9861(54)90309-4

Cited by 50 publications

(8 citation statements)

References 10 publications

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“…Fatty acids were eluted with 1.5 liters 50:50 chloroform-ethanol. Following evaporation the bile acid fraction was placed on a column [29] and the bile acids were separated by elution with increasing proportions of benzene in petroleum ether (16). Five milliliters of benzene were added to each tube fraction; they were evaporated and the acids were titrated with 0.1 N sodium hydroxide with phenol red as the indicator.…”

Section: Methodsmentioning

confidence: 99%

Primary and Secondary Bile Acids in Meconium

et al. 1971

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ExtractMeconium, newborn stool, and fetal bile were examined for bile acids by the techniques of gas liquid chromatography, thin layer chromatography and colorimetric spectrometry. Cholic, 1 chenodeoxycholic, deoxycholic, and lithocholic acid were detected in meconium. Secondary bile acids were not present in significant amounts in either stool or fetal bile from newborns. During fetal development more chenodeoxycholate than cholate is formed. The presence of secondary bile acids in meconium is suggestive of maternal-to-fetal transfer via the placenta.

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Section: Methodsmentioning

confidence: 99%

Primary and Secondary Bile Acids in Meconium

et al. 1971

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“…Purification of the tritiated product was carried out using Celite column partition chromatography (6). After chromatography, repeated crystallizations demonstrated a constant SA of 5.9 mCi/,umole.…”

Section: Methodsmentioning

confidence: 99%

“…Initial separation of the bile acids in the bile collected from these patients after hydrolysis was done using Celite column partition chromatography as described previously (3,6). (3) was rechromatographed on a smaller Celite column and counted for radioactivity.…”

Section: Methodsmentioning

confidence: 99%

The formation and metabolism of 3α,7α-dihydroxy-5β-cholestan-26-oic acid in man

Hanson¹

1971

J. Clin. Invest.

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“…The small amounts of the latter ingested with the diet did not reduce appreciably the specific radioactivity of fecal sterols. The values for bile acids were those of deoxycholic acid isolated from fecal SF by reverse-phase chromatography on Celite columns according to the method of Mosbach, Zomzely, and Kendall (11). Before chromatography it was necessary to remove fatty acids by partition between 70% aqueous methanol and heptane.…”

Section: Methodsmentioning

confidence: 99%

Sterol and bile acid excretion in man and the effects of dietary fat.

Avigan¹,

Steinberg²

1965

J. Clin. Invest.

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It is now well established that diets rich in saturated fatty acids lead to high levels of serum cholesterol, whereas diets rich in unsaturated fatty acids tend to be associated with lower levels. The mechanism by which these changes in serum cholesterol are induced by dietary fat is not known. One possible explanation for these effects would be either inhibition of cholesterol biosynthesis by unsaturated fats or stimulation of synthesis by saturated fats. The effects on synthesis, if any, could, in turn, be either the result of a direct action of the dietary fat on the biosynthetic system or of a secondary adjustment to any change in the rate of oxidation and excretion of cholesterol and its metabolites. Alternatively, changes in dietary fat might not affect the turnover rate, but cause a shift of part of the serum cholesterol pool, either from or into tissue compartments.Finally, the changes in serum cholesterol might reflect a primary effect not on sterol metabolism per se, but on the metabolism of lipoproteins, direct or mediated through effects on the metabolism of one or several of the moieties making up the macromolecule. These might act singly or in combination.In the present studies we have administered cholesterol-4-14C to subjects fed liquid formula diets containing saturated or unsaturated fat and subsequently measured the specific radioactivity of plasma cholesterol as a function of time. Simultaneously, the fecal excretion of sterol and bile acid was measured by a radioactivity balance method.

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Separation of bile acids by column-partition chromatography

Cited by 50 publications

References 10 publications

Primary and Secondary Bile Acids in Meconium

Primary and Secondary Bile Acids in Meconium

The formation and metabolism of 3α,7α-dihydroxy-5β-cholestan-26-oic acid in man

Sterol and bile acid excretion in man and the effects of dietary fat.

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