Separation of bacteria by capillary electrophoresis

Abstract

Separation of bacteria by capillary electrophoresisDifferences in the surface charges of bacteria can be exploited for their separation by capillary electrophoresis. Because of their low electrophoretic mobility, the separation is not always easy to perform, especially in the presence of the electroosmotic flow. Elimination of electroosmotic flow by capillary wall modification with c-(trimethoxysilyl)propyl methacrylate followed by acrylamide bonding permits separation over a distance of 8.5 cm.

Evaluation of Intact Cell Matrix-Assisted Laser Desorption/Ionization Timeof- Flight Mass Spectrometry for Capillary Electrophoresis Detection of Controlled Bacterial Clumping

“…Unfortunately, this technique bases on conventional and restricted UV-or diode array detection (DAD) and requires long time for optimization of separation process [6,7]. Solving the problem of detection, raising selectivity and reducing the time to identify microorganisms is possible with the use of intact cell matrix-assisted laser-desorption/ionization (IC MALDI) time-of-flight mass spectrometry (TOF MS) method [1][2][3][4][5]8].…”

Determination of pathogenic bacteria by CZE with surface‐modified capillaries

“…Buszewski and coworkers [37,38] investigated modification of the internal capillary surface using three different monomers: acrylamide, trimethylchlorosilane and divinylbenzene (DVB). Such approach results in suppression of EOF and prevents the adsorption of bacteria to the capillary wall.…”

Detection of microbial food contaminants and their products by capillary electromigration techniques

“…Buszewski et al [51,52] investigated microbial separations in modified CE capillaries. A first attempt involved the use of acrylamide-modified capillary and UV detection to determine the electrophoretic mobilities of E. coli, Pseudomonas fluorescens, Bacillus cereus and Proteus vulgaris.…”

“…A first attempt involved the use of acrylamide-modified capillary and UV detection to determine the electrophoretic mobilities of E. coli, Pseudomonas fluorescens, Bacillus cereus and Proteus vulgaris. Under normal polarity and injecting a microbe mixture into the shorter part of the capillary, it was possible to separate the microorganisms in seven minutes [51]. The same group also separated a mixture of other five bacteria using trimethylchlorosilane-and divinylbenzenemodified capillaries [52].…”