Separation of aracytidine and cytidine by capillary electrophoretic techniques

Abstract

Aracytidine (cytarabine, 1-beta-D-arabinofuranosylcytosine) is a synthetic analog of cytidine in which ribose is substituted by arabinose; it is used as a drug for the treatment of leukemia. A fast and reliable capillary electrophoretic method for the analysis of cytarabine and cytidine is described. The procedure utilizes the interactions with sodium dodecyl sulfate (SDS) micelles and borate, present in the background electrolyte, for the mobilization and selective separation of the analytes. The detection is carried out by UV absorbance at 275 nm. The method was applied both to pharmaceutical preparations and human serum. Analysis of an untreated serum requires 15 min; the detection limit is 0.8 microgram/mL and the relative standard deviation (RSD) is 5.3%.

Antineoplastic drugs and their analysis: a state of the art review

“…The limitations previously described for ion pairing chromatography, ion exchange chromatography or normal phase LC were all tackled with HILIC, and this is why this analytical technique was successfully applied for the analysis of pyrimidine analogues. Various CE-UV methods have also been suggested for the analysis of 5FU 39,[105][106][107][108]109 and cytarabine, [110][111][112] since CE works with any type of ionisable substance, whatever the polarity. LOD of 1.7 ng mL −1 was reached for the analysis of 5FU in effluents using an SPE-CE-UV method.…”

Simultaneous separation and determination of Tarabine PFS and Adriblastine using micellar electrokinetic chromatography and high performance liquid chromatography. Application to some biological fluids

“…In the literature, there are several reports on the determination of Tarabine PFS individually or with other compounds by CE [7,8] or by HPLC [9 -11]. These methods depend on the determination of parent compound with its metabolites or with synthetic analogs.…”

Osteryoung square wave stripping voltammetry at mercury film electrode for monitoring ultra trace levels of Tarabine PFS and its interaction with ssDNA

“…Several analytical methods for the determination of Tarabine PFS have been reported including UVspectrophotometry and polarography [8][9][10][11][12], derivative spectroscopy [13,14], gas chromatography [15], high performance liquid chromatography (HPLC) [16][17][18][19][20][21][22] and capillary electrophoresis techniques [23]. Also, Tarabine PFS and other pyrimidine derivatives have been detected by radio-enzymes [24], radioimmunological [25] and microbiological methods of analysis [26,27].…”