Separation of a Breast Cancer Cell Line from Human Blood Using a Quadrupole Magnetic Flow Sorter

Abstract

We have developed a quadrupole magnetic flow sorter (QMS) to facilitate high-throughput binary cell separation. Optimized QMS operation requires the adjustment of three flow parameters based on the immunomagnetic characteristics of the target cell sample. To overcome the inefficiency of semiempirical operation/optimization of QMS flow parameters, a theoretical model of the QMS sorting process was developed. Application of this model requires measurement of the magnetophoretic mobility distribution of the cell sample by the cell tracking velocimetry (CTV) technique developed in our laboratory. In this work, the theoretical model was experimentally tested using breast carcinoma cells (HCC1954) overexpressing the HER-2/neu gene, and peripheral blood leukocytes (PBLs). The magnetophoretic mobility distribution of immunomagnetically labeled HCC1954 cells was measured using the CTV technique, and then theoretical predictions of sorting recoveries were calculated. Mean magnetophoretic mobilities of (1-3) x 10(-4) mm(3)/(T A s) were obtained depending on the labeling conditions. Labeled HCC1954 cells were mixed with unlabeled PBLs to form a "spiked" sample to be separated by the QMS. Fractional recoveries of cells for different flow parameters were examined and compared with theoretical predictions. Experimental results showed that the theoretical model accurately predicted fractional recoveries of HCC1954 cells. High-throughput (3.29 x 10(5) cells/s) separations with high recovery (0.89) of HCC1954 cells were achieved.

Blood progenitor cell separation from clinical leukapheresis product by magnetic nanoparticle binding and magnetophoresis

“…The effects of flow rates on the QMS performance have been theoretically analyzed and experimentally investigated in previous studies (Hoyos et al, 2000(Hoyos et al, , 2002Nakamura et al, 2001;Williams et al, 1999). This manuscript focuses on the utilization of outlet flow rate control to collect the CD34þ cells with different purity and recovery.…”

Dielectrophoresis-Based Sample Handling in General-Purpose Programmable Diagnostic Instruments

“…In this case, the magnetic force depends only on the magnetic field gradient. While (8) and (9) apply to the magnetic capture of cells by immunomagnetic cell selection [85], the goal in DEP-MAP-FFF is not to trap the cells. In this method, a DEP-FFF separation chamber sits on a periodically varying magnetically polarizable layer that itself overlays a flat magnet (Fig.…”

Comparison of two immunomagnetic separation technologies to deplete T cells from human blood samples

“…It has been previously demonstrated that using both the batch Miltenyi Biotec MACS system or the flow-through, QMS system performance is partially based on the degree to which a cell is immunomagnetically labeled, quantitatively referred to as a cell's magnetophoretic mobility Nakamura et al, 2001;Williams et al, 1999). In addition, the manufacturer of the MACS system states that the MS column can be used for depletion separations if the target cells are ''strongly magnetically labeled'' (Miltenyi Biotec Catalog, Subsection 14.3).…”