Dielectrophoresis-Based Sample Handling in General-Purpose Programmable Diagnostic Instruments

Gascoyne, Peter R. C.; Vykoukal, Jody

doi:10.1109/jproc.2003.820535

Cited by 279 publications

(168 citation statements)

References 107 publications

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“…As different cell types exhibit dissimilar electrical properties, DEP can be used to sort between cell types, or even between widely varying cells of the same type [16], [19], [20]. This property is useful for cellular manipulation, in which heterogeneous mixtures of cells are common.…”

Section: B Cell Discrimination Using Oetmentioning

confidence: 99%

“…Typically, microfabricated metal electrodes are used to create electric field gradients that are sufficient to trap microand nanoscale particles [15]- [17]. Dielectrophoretic manipulation is useful for a variety of functions, including cell trapping [16]- [18] and cell sorting [16], [19], [20]. However, the fixed electrode patterns limit the flexibility of individual devices, and make it difficult to isolate a particular cell from a population.…”

Section: Introductionmentioning

confidence: 99%

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Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers

Ohta

Chiou

Phan³

et al. 2007

IEEE J. Select. Topics Quantum Electron.

121

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Abstract-Optoelectronic tweezers (OET) provide a powerful tool for the manipulation of micro-and nanoparticles. The OET device produces an optically controlled dielectrophoretic force, allowing complex dynamic manipulation patterns using light intensities up to 100 000 times lower than that of optical tweezers. Using OET, we demonstrate the separation of live and dead human B cells, and the separation of HeLa and Jurkat cells. We also present, for the first time, a modified single-sided OET device that promises to facilitate the integration of OET and microfluidics. Unlike standard OET, this single-sided OET device produces electric fields that are oriented parallel to the plane of the device. We demonstrate the manipulation of polystyrene beads using this new single-sided OET device, and discuss its capabilities. Index Terms-Dielectrophoresis (DEP), optical tweezers, optically induced dielectrophoresis, optoelectronic tweezers (OET).

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Section: B Cell Discrimination Using Oetmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers

Ohta

Chiou

Phan³

et al. 2007

IEEE J. Select. Topics Quantum Electron.

121

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“…We use high frequencies ͑GHz͒ for capacitance measurements to avoid interference and variability due to effects such as electrical double layers, 6,17,19 ionic conduction, and other forms of dielectric variation in materials with frequency, which are dominant at frequencies below 200 MHz. 20 The use of high frequencies also opens the door for lower frequencies ͑MHz͒ to be used for other purposes such as simultaneous electromanipulation of biological materials.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic electromanipulation with capacitive detection for the mechanical analysis of cells

et al. 2008

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The mechanical behavior of cells offers insight into many aspects of their properties. We propose an approach to the mechanical analysis of cells that uses a combination of electromanipulation for stimulus and capacitance for sensing. To demonstrate this approach, polystyrene spheres and yeast cells flowing in a 25 m ϫ 100 m microfluidic channel were detected by a perpendicular pair of gold thin film electrodes in the channel, spaced 25 m apart. The presence of cells was detected by capacitance changes between the gold electrodes. The capacitance sensor was a resonant coaxial radio frequency cavity ͑2.3 GHz͒ coupled to the electrodes. The presence of yeast cells ͑Saccharomyces cerevisiae͒ and polystyrene spheres resulted in capacitance changes of approximately 10 and 100 attoFarad ͑aF͒, respectively, with an achieved capacitance resolution of less than 2 aF in a 30 Hz bandwidth. The resolution is better than previously reported in the literature, and the capacitance changes are in agreement with values estimated by finite element simulations. Yeast cells were trapped using dielectrophoretic forces by applying a 3 V signal at 1 MHz between the electrodes. After trapping, the cells were displaced using amplitude and frequency modulated voltages to produce modulated dielectrophoretic forces. Repetitive displacement and relaxation of these cells was observed using both capacitance and video microscopy.

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“…While various methods for handling cells in suspensions have been proposed, each technique has draw-backs that limit its potential. Methods using electric fields for trapping and exposing mammalian cells to new reagents [4][5][6] are dependent on the solution for cell suspension and on the cell type. Optical manipulation of relatively large mammalian cells 7 can be laborious, expensive, and cannot be easily scaled up, while the use of mechanical structures [8][9][10] is usually irreversible, since once cells are mechanically trapped they cannot be easily released.…”

Section: Introductionmentioning

confidence: 99%

Cell handling using microstructured membranes

Irimia

Toner

2006

Lab Chip

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Gentle and precise handling of cell suspensions is essential for scientific research and clinical diagnostic applications. Although different techniques for cell analysis at the micro-scale have been proposed, many still require that preliminary sample preparation steps be performed off the chip. Here we present a microstructured membrane as a new microfluidic design concept, enabling the implementation of common sample preparation procedures for suspensions of eukaryotic cells in lab-on-a-chip devices. We demonstrate the novel capabilities for sample preparation procedures by the implementation of metered sampling of nanoliter volumes of whole blood, concentration increase up to three orders of magnitude of sparse cell suspension, and circumferentially uniform, sequential exposure of cells to reagents. We implemented these functions by using microstructured membranes that are pneumatically actuated and allowed to reversibly decouple the flow of fluids and the displacement of eukaryotic cells in suspensions. Furthermore, by integrating multiple structures on the same membrane, complex sequential procedures are possible using a limited number of control steps.

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Dielectrophoresis-Based Sample Handling in General-Purpose Programmable Diagnostic Instruments

Cited by 279 publications

References 107 publications

Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers

Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers

Microfluidic electromanipulation with capacitive detection for the mechanical analysis of cells

Cell handling using microstructured membranes

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