Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers

Ohta, Aaron T.; Chiou, Pei-Yu; Phan, H.L.; Sherwood, S.W.; Yang, Joon Mo; Lau, Aldrich N. K.; Hsu, Hsan-Yin; Jamshidi, Arash; Wu, M.C.

doi:10.1109/jstqe.2007.893558

Cited by 121 publications

(92 citation statements)

References 29 publications

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“…We note that G. B. Lee's (a co-author of this paper) group has already demonstrated the increase of ODEP force due to different image colors projected onto the surface of an ODEP chip in [18], however only a qualitative explanation was provided in their prior work. To the best of our knowledge, this paper is the first to present a theoretical model with experimental validation of how the ODEP force varies with the variation of optical spectrum of projected images on ODEP chips.…”

Section: Introductionmentioning

confidence: 88%

“…In addition, OET devices that generate light-induced AC electroosmosis (ACEO) have been widely used in the manipulation of nanoparticles [12], dynamic control of local chemical concentration in a fluid [13], measurement of molecular diffusion coefficient [14], assembly of 2D colloidal crystals [15], detection of human tumor markers [16], and rapid and selective concentration of microparticles [17]. Furthermore, the separation of micro-particles of different diameters using different light colors, light intensities [18,19], and widths of virtual line electrodes [19] has also been experimentally validated.…”

Section: Introductionmentioning

confidence: 99%

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Optical Spectrum and Electric Field Waveform Dependent Optically-Induced Dielectrophoretic (ODEP) Micro-Manipulation

et al. 2012

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Abstract:In the last seven years, optoelectronic tweezers using optically-induced dielectrophoretic (ODEP) force have been explored experimentally with much success in manipulating micro/nano objects. However, not much has been done in terms of in-depth understanding of the ODEP-based manipulation process or optimizing the input physical parameters to maximize ODEP force. We present our work on analyzing two significant influencing factors in generating ODEP force on a-Si:H based ODEP chips: (1) the waveforms of the AC electric potential across the fluidic medium in an ODEP chip based microfluidic platform; and (2) optical spectrum of the light image projected onto the ODEP chip. Theoretical and simulation results indicate that when square waves are used as the AC electric potential instead of sine waves, ODEP force can double. Moreover, numerical results show that ODEP force increases with increasing optical frequency of the projected light on an ODEP chip following the Fermi-Dirac function, validating that the optically-induced dielectrophoresis force depends strongly on the electron-hole carrier generation phenomena in optoelectronic materials. Qualitative experimental results that validate the numerical results are also presented in this paper. OPEN ACCESSMicromachines 2012, 3 493

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Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Optical Spectrum and Electric Field Waveform Dependent Optically-Induced Dielectrophoretic (ODEP) Micro-Manipulation

et al. 2012

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“…Without illumination, the majority of applied AC voltage drops across the a-Si:H layer. However, in the presence of light, the partial voltage on liquid layer is significantly increased due to approximately 3-4 orders of magnitude increase of the conductivity of a-Si:H when illuminated, which arises from the creation of electron-hole pairs [54,61]. A spatial electric field gradient without phase nonuniformity is created near the illuminated area when an AC bias is applied between the two electrodes ( Figure 2.3a).…”

Section: Oek Chip: Operational Principle and Designmentioning

confidence: 99%

Micro and Nano Manipulation and Assembly by Optically Induced Electrokinetics

Lai

et al. 2015

Micro‐ and Nanomanipulation Tools

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IntroductionIn the past 25 years, integrated circuit (IC) and microelectromechanical systems (MEMSs) technologies have moved much beyond the microdomain, and into the submicro-, nanoscopic, and, even, the molecular scales. Today, we have the capability to fabricate, manipulate, and assemble matters at the micro-, nano-, or molecular scales. This progress into "seeing" and manipulating matters that are smaller than visible light wavelength scale is impacting a range of fields including semiconductor physics, biological studies, pharmaceutical development, and many other scientific and technological applications. A range of new methods to generate physical forces have been developed in the process. For example, mechanical forces can now be applied to micro-, nano-, and biological entities using atomic force microscope probes [1], microgrippers [2], or pipettes [3]. However, disadvantages of these approaches, including inflection of mechanical damages on objects being manipulated and their relatively low manipulation speed, make manipulation of large quantities of objects difficult. In order to address these disadvantages, noncontact or noninvasive methods for micro-, nano-, and biological manipulation have also been explored in the past two decades.Manipulation devices based on magnetic forces [4], acoustic waves [5,6], hydrodynamic flows [7], light waves [8], or electrokinetic forces [9] are among the major noninvasive technologies available today. Each of them has its own advantages and disadvantages. Methods based on magnetic fields need premodification on objects with magnetic beads which constrains their application domain. In addition, movement controlled by permanent magnets is not precise enough for many proposed applications. Also, the high resistance of the magnetic coil generates significant heat during manipulation [10]. Acoustic traps show good selectivity but cannot achieve parallel manipulation of single entities. Microfluidic devices exploiting hydrodynamic flows are suitable for cell population sorting, but have difficulties in trapping or controlling specific single objects.Electrokinetic forces generated from patterned electrodes capable of inducing the desired spatial distribution of electric field have been exploited to control

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“…Due to the flexibility of light pattern, the nonuniform electric field induced by illuminating method is able to manipulate the microparticle within the region which the light can cover. Polystyrene particles [10,13], biological sample [10,11,[13][14][15] DNA [16,17], and other microparticles [18][19][20][21] are able to be manipulated by this optoelectronic approach.…”

Section: Introductionmentioning

confidence: 99%

Moldless PEGDA-Based Optoelectrofluidic Platform for Microparticle Selection

Yang

Liu³

et al. 2011

Advances in OptoElectronics

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This paper reports on an optoelectrofluidic platform which consists of the organic photoconductive material, titanium oxide phthalocyanine (TiOPc), and the photocrosslinkable polymer, poly (ethylene glycol) diacrylate (PEGDA). TiOPc simplifies the fabrication process of the optoelectronic chip due to requiring only a single spin-coating step. PEGDA is applied to embed the moldless PEGDA-based microchannel between the top ITO glass and the bottom TiOPc substrate. A real-time control interface via a touch panel screen is utilized to select the target 15 μm polystyrene particles. When the microparticles flow to an illuminating light bar, which is oblique to the microfluidic flow path, the lateral driving force diverts the microparticles. Two light patterns, the switching oblique light bar and the optoelectronic ladder phenomenon, are designed to demonstrate the features. This work integrating the new material design, TiOPc and PEGDA, and the ability of mobile microparticle manipulation demonstrates the potential of optoelectronic approach.

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Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers

Cited by 121 publications

References 29 publications

Optical Spectrum and Electric Field Waveform Dependent Optically-Induced Dielectrophoretic (ODEP) Micro-Manipulation

Optical Spectrum and Electric Field Waveform Dependent Optically-Induced Dielectrophoretic (ODEP) Micro-Manipulation

Micro and Nano Manipulation and Assembly by Optically Induced Electrokinetics

Moldless PEGDA-Based Optoelectrofluidic Platform for Microparticle Selection

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