Separation of [3H]inositol monophosphates and [3H]inositol on silica gel glass-fiber sheets

Hokin-Neaverson, Mabel; Sadeghian, Kenneth

doi:10.1016/s0021-9673(76)80031-3

Cited by 39 publications

(12 citation statements)

References 6 publications

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“…One ml fractions were recovered and radioactivity was determined by scintillation counting. Peak areas were determined by triangulation (Hokin‐Neaverson and Sadeghian, 1976). Results were expressed as a percentage of the total radioactivity incorporated (1st plus 2nd peaks).…”

Section: Methodsmentioning

confidence: 99%

“…Results were expressed as a percentage of the total radioactivity incorporated (1st plus 2nd peaks). In order to determine the absence of ( 3 H)‐MI in the eluted peaks of InsP, chromatography in silica gel 60 F254 sheets (Merck & Co, Inc., Whitehouse Station, NJ) was performed using propan‐2‐ol‐6,NH 4 (14:5) as the developing solvent (Hokin‐Neaverson and Sadeghian, 1976). Spots were located by spraying with freshly prepared 0.1% ferric chloride in ethanol followed, after air drying with 1% sulphosalicylic acid in ethanol.…”

Section: Methodsmentioning

confidence: 99%

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Dynamic cell shapes and contacts in the developing Drosophila retina are regulated by the Ig cell adhesion protein hibris

Grillo-Hill

Wolff

2009

Developmental Dynamics

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Cell shapes and contacts are dynamically regulated during organogenesis to enable contacts with relevant neighboring cells at appropriate times. During Drosophila larval eye development, an apical contact is established between one pair of non-neuronal cones cells, precluding contact between the opposing pair. Concurrent with changes in cell shape, these contacts reverse in early pupal life. The reversal in cone cell contacts occurs in a posterior to anterior gradient across the eye, following the developmental gradient established in the larval eye imaginal disc. Hibris (Hbs), an Immunoglobulin cell adhesion molecule homologous to vertebrate Nephrin, is required for cone cell morphogenesis. In hbs null mutants, a majority of cone cells fail to both establish wild-type contacts and achieve mature cone cell shapes. hbs acts cell autonomously in the cone cells to drive these changes. The work presented here indicates hbs contributes to the remodeling of cell contacts and cell shapes throughout development. Developmental Dynamics 238: 2223-2234, 2009.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Dynamic cell shapes and contacts in the developing Drosophila retina are regulated by the Ig cell adhesion protein hibris

Grillo-Hill

Wolff

2009

Developmental Dynamics

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“…Results corresponding to the second peak, were expressed as a percentage of the total radioactivity incorporated (1st plus 2nd peaks). In order to determine the absence of [3H]-myo-inositol in the eluted peaks of IPs, chromatography in silica gel 60 F254 sheets (Merck) was performed using propan-2-ol/6, NH4 (14: 5) as the developing solvent (Hokin-Neaverson & Sadeghian, 1976). Spots were located by spraying with freshly prepared 0.1% ferric chloride in ethanol followed, after airdrying, with 1% sulphosalicylic acid in ethanol.…”

Section: Atrial Preparation For Contractilitymentioning

confidence: 99%

Endogenous nitric oxide signalling system and the cardiac muscarinic acetylcholine receptor‐inotropic response

Sterin‐Borda

Echagüe

Leirós

et al. 1995

British J Pharmacology

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1 In this paper we have determined the different signalling pathways involved in muscarinic acetylcholine receptor (AChR)-dependent inhibition of contractility in rat isolated atria. 2 Carbachol stimulation of M2 muscarinic AChRs exerts a negative inotropic response, activation of phosphoinositide turnover, stimulation of nitric oxide synthase and increased production of cyclic GMP. 3 Inhibitors of phospholipase C, protein kinase C, calcium/calmodulin, nitric oxide synthase and guanylate cyclase, shifted the dose-response curve of carbachol on contractility to the right. These inhibitors also attenuated the muscarinic receptor-dependent increase in cyclic GMP and activation of nitric oxide synthase. In addition, sodium nitroprusside, isosorbide, or 8-bromo cyclic GMP, induced a negative inotropic effect, increased cyclic GMP and activated nitric oxide synthase. 4 These results suggest that carbachol activation of M2 AChRs, exerts a negative inotropic effect associated with increased production of nitric oxide and cyclic GMP. The mechanism appears to occur secondarily to stimulation of phosphoinositides turnover via phospholipase C activation. This in turn, triggers cascade reactions involving calcium/calmodulin and protein kinase C, leading to activation of nitric oxide synthase and soluble guanylate cyclase.

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“…These latter metabolites can be isolated and resolved by the use of ion exchange, thin-layer chromatography, paper chromatography, and silica gel glass-fiber sheets (Hokin-Neaverson and Sadeghian, 1976;KochKallnbach and Diringer, 1977;Diringer et ai., 1977). Inositol pentaphosphate has long been recognized as a predominant organic phosphate in the erythrocytes of most avian species (Johnson and Tate, 1969).…”

Section: Inositol and Its Biological Formsmentioning

confidence: 99%

The Nutritional Significance, Metabolism, and Function of myo-Inositol and Phosphatidylinositol in Health and Disease

Holub

1982

Advances in Nutritional Research

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Separation of [3H]inositol monophosphates and [3H]inositol on silica gel glass-fiber sheets

Cited by 39 publications

References 6 publications

Dynamic cell shapes and contacts in the developing Drosophila retina are regulated by the Ig cell adhesion protein hibris

Dynamic cell shapes and contacts in the developing Drosophila retina are regulated by the Ig cell adhesion protein hibris

Endogenous nitric oxide signalling system and the cardiac muscarinic acetylcholine receptor‐inotropic response

The Nutritional Significance, Metabolism, and Function of myo-Inositol and Phosphatidylinositol in Health and Disease

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