Separation and structural characterization of a synthetic cannabinoid found in a herbal product using off-line LC-DAD-NMR

Rapid Chemical Characterisation of Stilbenes in the Root Bark of Norway Spruce by Off-line HPLC/DAD-NMR

“…The fractions are then transferred from the storage loops to vials, and the same eluates from multiple injections can be pooled together from multiple injections. In this way, the pooled fractions can be easily concentrated to obtain a sufficient amount of analyte for an off‐line HPLC/DAD–NMR analysis (Koskela et al , ).…”

Application of comprehensive NMR‐based analysis strategy in annotation, isolation and structure elucidation of low molecular weight metabolites of Ricinus communis seeds

“…A portion of a 1.5 mL of the 2% deuterated acetic acid extract of seed was concentrated to approximately 500 μL under mild nitrogen flow. This concentrate was used for optimising HPLC conditions and for preparative separation of the extract components with the procedure described in more detail elsewhere (Koskela et al ., ; Koskela et al ., ). In short, chromatographic separation was done on an Agilent 1200 HPLC system (Santa Clara, CA, USA), consisting of a solvent degasser, a binary pump, an autosampler, a column oven and a diode array detector.…”

An overview of recent developments in the analytical detection of new psychoactive substances (NPSs)

“…59 In addition to the identification of the chemical components contained within the Spice product range there is a need to understand the effects of the synthetic cannabinoids on the human metabolism. 72 Different analytical approaches on Spice and related products have been reported [73][74][75][76][77][78] with literature reporting the presence of new cannabimimetic compounds. Using LC-MS and GC-MS, two main monohydroxylated metabolites were identified which are almost completely glucuroconjugated with minor metabolites such as N-despentyl hydroxy-, carboxy-, dihydroxy-, and reduced di-and trihydroxy-metabolites.…”

“…72 The authors observed that there are two main metabolites that are valuable for detection of JWH-018 in post-administration urine and LC-MS is a more useful technique as minor metabolites can also be analysed to support analytical findings. 72 Different analytical approaches on Spice and related products have been reported [73][74][75][76][77][78] with literature reporting the presence of new cannabimimetic compounds. 60,79,80 Following this pioneering work, there has been a pursuit of studying synthetic cannabinoids in urine.…”